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Colorimetric estimation of thiamin by the ninhydrin reaction

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Summary

The compound formed by the reaction between thiamin and ninhydrin in a weakly acid medium is of use in the colorimetric estimation of the concentration of thiamin in aqueous solution.

The optimal conditions for this reaction were studied and established.

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Literature Cited

  1. A. A. Dmitrovskii, Vitamin Resources and Their Utilization, Vol. 3, p. 100. Moscow, 1955 [in Russian].

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  2. P. Gyorgy and S. H. Rubin, The Biochemistry and Physiology of Vitamins, Vol. 4, p. 7. Moscow, 1951 [Russian translation].

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  3. B. C. P. Jansen Rec. trav. chim., 1936, v. 55, p. 1046.

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Authors and Affiliations

  1. Vitebsk Medical Institute and the Laboratory of the Regional Hospital, Vitebsk, USSR

    Yu. M. Ostrovskii & M. A. Gvozdeva

Authors
  1. Yu. M. Ostrovskii
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  2. M. A. Gvozdeva
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Ostrovskii, Y.M., Gvozdeva, M.A. Colorimetric estimation of thiamin by the ninhydrin reaction. Bull Exp Biol Med 48, 1433–1434 (1959). https://doi.org/10.1007/BF00788365

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  • DOI: https://doi.org/10.1007/BF00788365

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