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Presence of serum albumin in normal human epidermis: Possible implications for the nutrition and physiology of stratified epithelia

Presence of albumin in epidermis

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Abstract

In this paper, using both immunofluorescence and protein biochemistry techniques, we present definitive evidence that plasma proteins such as albumin are present within normal human epidermis. This result confirms several previous reports supporting the idea that relatively large molecules can diffuse through the epidermal basement membrane into epidermis. Our results bring new insights for discussing how hydrophobic ligands or drugs present in the bloodstream and bound to plasmatic carriers can reach epidermal cells of all layers.

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Abbreviations

CHAPS:

3-[3-cholamidopropyl dimethylammonio] propane sulfonate

kD:

kilodaltons

BSA:

bovine serum albumin

2ME:

2-mercaptoethanol

DTT:

dithiothreitol

SDS:

sodium dodecyl sulfate

pI:

isoelectric point

Mw:

molecular weight

Tris:

Tris-(hydroxymethyl) aminomethane

1D:

one dimensional

2D:

two dimensional

PAGE:

poly acrylamide gel electrophoresis

MEM:

Minimal Eagle's Medium

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Author information

Authors and Affiliations

  1. Department of Biology, Ecole Normale Supérieure, CNRS URA 686, Paris, France

    Thierry Rabilloud

  2. Department of Cell Biology, Centre International de Recherches Dermatologiques (CIRD), Sophia Antipolis, Valbonne, France

    Daniel Asselineau

  3. CIRD, Sophia Antipolis, 06565, Valbonne Cédex, France

    Michel Darmon

Authors
  1. Thierry Rabilloud
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  2. Daniel Asselineau
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  3. Michel Darmon
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Rabilloud, T., Asselineau, D. & Darmon, M. Presence of serum albumin in normal human epidermis: Possible implications for the nutrition and physiology of stratified epithelia. Mol Biol Rep 13, 213–219 (1988). https://doi.org/10.1007/BF00788173

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  • DOI: https://doi.org/10.1007/BF00788173

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