Molecular Biology Reports

, Volume 13, Issue 4, pp 197–206 | Cite as

Plasma membrane and cytoskeletal constituents inLeishmania mexicana

  • F. Dagger
  • I. Dunia
  • A. G. Hernandez
  • L. A. Pradel
  • E. L. Benedetti
Article

Abstract

The presence and the localization of actin, spectrin and ankyrin are studied by immunofluorescence and immunoblotting inLeishmania mexicana promastigotes growing in vitro.

These proteins, amphitropic in nature, coexist both in soluble and insoluble forms. Our results demonstrate that the Triton insoluble form of these proteins constitutes beside tubulin the cytoskeletal scaffold of promastigotes in close association with the plasma membrane, the axoneme and the basal body of the parasite.

Key words

Leishmania plasma membrane cytoskeleton immunofluorescence immunoblot 

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Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • F. Dagger
    • 1
  • I. Dunia
    • 2
  • A. G. Hernandez
    • 1
  • L. A. Pradel
    • 3
  • E. L. Benedetti
    • 2
  1. 1.Department of Cell Biology, Faculty of SciencesUniversidad Central de VenezuelaCaracasVenezuela
  2. 2.Institut Jacques Monod du CNRSUniversité Paris VIIparis Cédex 05France
  3. 3.Institut de Biologie Physico-ChimiqueParisFrance

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