Summary
Spherical bodies resembling Weibull's protoplasts are formed when certain bacteria (B. megatherium Salmonella gallinarum,B. mesentericus) are incubated with lysozyme or penicillin in the presence of 10% sucrose, as a stabilizer. The presence of mitotic figures in some of the protoplasts suggests that they are capable of development and regeneration.
TheS. gallinarum protoplasts showed some respiratory activity, although much less than the intact cells.B. megatherium protoplasts did not respire.
Similar content being viewed by others
Literature Cited
A. Oparin, N. Gel'man and L. Zhukova, Doklady Akad. Nauk SSSR 105, 1036 (1955).
M. Beljanski, Biophys. Acta. 1954, 15, pp. 425–431.
M. Bridoux and J. Hanotier, J. Bioch. Biophys. Acta. 1956, 22, pp. 103–110.
I. Lederberg, Proc. Nat. Acad. sci. USA. 1956, 42, N. 9, pp. 574–577.
H. Maher and D. Fraser, Bioch. Biophys. Acta. 1956, 22, pp. 197–199.
K. McQuillen, Bioch. Biophys. Acta. 1955, 17, pp. 382–390.
K. McQuillen, J. Gener. Microbiol. 1955, 13, N. 1, p. IV.
K. McQuillen, Bioch. Biophys. Acta, 1955, 18, pp. 458–461.
C. Penso, Problemes actuels de virologie. Paris, 1954, pp. 107–130.
M. Salton, J. gener. Microbiol. 1955, 13, N. 1, pp. IV-V.
M. Salton, Nature 1952, 170, 746.
M. Salton and K. McQuillen, Bioch. Biophys. Acta 1955, 17, pp. 465–472.
K. Sester, J. Amer. Chem. Soc. 1953, 75, pp. 5448–5449.
J. Sharp, W. Higmans and L. Dienes, J. exper. med. 1957, v. 105, N. 2, pp. 153–159.
C. Weibull, Bacter. Anatomy. The 6th Symposium of the Soc. for General Microbiology, 1956. pp. 111–126.
C. Weibull, J. Bacter. 1953, 66, pp. 688–695.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gorodskaia, G.I., Miagkaia, G.L. Preliminary results of a study of bacterial protoplasts. Bull Exp Biol Med 46, 990–995 (1958). https://doi.org/10.1007/BF00787342
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00787342