Bulletin of Experimental Biology and Medicine

, Volume 82, Issue 2, pp 1202–1205 | Cite as

Physicochemical properties of a mitogenetic factor from human lymphocytes induced by phytohemagglutinin

  • N. N. Voitenok
  • N. V. Varivotskaya
  • P. P. Murzenok
  • N. D. Potemkina
Immunology and Microbiology


The physiochemical properties of a mitogenetic factor (MF) secreted by human lymphocytesin vitro under the influence of phytohemagglutinin were studied. Treatment of the culture medium with proteases was shown to reduce activity of the MF sharply. On gel chromatography on Sephadex and Biogel columns all the mitogenetic activity was concentrated in fractions corresponding to a molecular weight of 20,000–30,000 (peak at 25,000). On disk electrophoresis in 5% polyacrylamide gel MF migrated in the region of α1 and α2 globulins and transferrin and was separated into several fractions. On isoelectric focusing the MF was arranged as three fractions within the pH range 4.5–8.3. The functional heterogeneity of the MF is postulated.

Key Words

Lymphokines phytohemagglutinin mitogenetic factor of human lymphocytes 


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Copyright information

© Plenum Publishing Corporation 1976

Authors and Affiliations

  • N. N. Voitenok
  • N. V. Varivotskaya
  • P. P. Murzenok
  • N. D. Potemkina

There are no affiliations available

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