Summary
A modification of Grabar's immunoelectrophoresis method is described. Electrophoresis is effected on slides placed in a special bridged cuvette which serves both to hold the agar poured over the slides and to carry out electrophoresis.
The amount of agar in the cuvette is somewhat in excess of the required. The overflowing agar is cut off with a razor sliding along specially arranged edges of the cuvette, thus, always ensuring a uniform thickness of the agar layer.
The first compartments for the introduction of the serum and the troughs for the antiserum are also strictly standard in size.
This is achieved with the aid of special patterns (templates) placed on the slides while the agar is poured. Their position is fixed by markers on the cuvette.
Immunoelectrophoregrams obtained by this method are characterized by their constancy.
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Literature Cited
P. Grabar, “Immunoelectrophoretic analysis of human serum proteins”, Biokhimiya, 22, 1–2, 49–59 (1957).
P. Grabar, Biochim. Biophys. Acta, 1955, v. 17, p. 67–74.
O. Ouchterlony, Lancet, 1949, v. 256, No. 6548, p. 346–348.
C. A. Williams, and P. Grabar, J. Immunol. 1955, v. 74, No. 2, p. 158–168.
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Vasileiskii, S.S. A modification of the method of immunoelectrophoresis of the serum proteins. Bull Exp Biol Med 48, 1285–1288 (1959). https://doi.org/10.1007/BF00785191
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DOI: https://doi.org/10.1007/BF00785191