Quantitative study of immune horse serum antibody a gainst Clostridium oedematiens by means of precipitation on paper or paper-fixed antigen

  • A. E. Gurvich
  • M. V. Ispolatovskaia
Microbiology and Immunity
  • 17 Downloads

Summary

Reaction of flocculation between the antitoxin ofCl,oedematiens and the corresponding immune horse serum was studied with the aid of precipitation on paper. When toxin and immune serum were placed on the same place on the paper, a flocculate appears, which unlike all the rest of the proteins is not washed off the paper by the veronal buffer. The fiocculate is precipitated at the zone of considerabic surplus of antitoxin. The quantity of precipitate is increased in proportion to the quantity of the added antigen up to the time when the whole quantity of the antigen is precipitated. Then the precipitate begins to dissolve in the surplus of the antigen. Data which were obtained by the method of precipitation on paper correspond to the results of determination of the quantity of the antibodies in the same serum with the aid of the antigen fixed on paper through diazoconnection and the residual haloidalkylate. This points to the possibillity of replacement of the biological method of determination of the antigens ofCl.oedematiens by the immunochemical method.

Keywords

Public Health Quantitative Study Horse Serum Serum Antibody Biological Method 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. [1]
    V.A. Blagoveshchenskii, I.V. Bulanova, N.V. Zakharova, M.V. Ispolatovskaia, I.P. Malorov and L.Ia. Marmalevskala, Reports of Proceedings of Inter-Institutional Scientific Conference on the Problem of “Anaerobiasis” [in Russian] (Moscow, 1956).Google Scholar
  2. [2]
    A.E. Gurvich, Biokhimlia 20, 5, 550 (1955).Google Scholar
  3. [3]
    A.E. Gurvich, Biokhimlia 22, 6, 1028–1034 (1957). Original Russian pagination. See C.B. translation.Google Scholar
  4. [4]
    V.A. Krestovnikova, O.A. Komkova, P.M. Dreltser and S.P. Zaeva, Zhur. Mikrobiol., Epidemiol., i Immunobiol. 11, 3 (1933).Google Scholar
  5. [5]
    E. Kabat and M. Heidelberger, J. Exper. Med. 66, 2, 229 (1937).Google Scholar
  6. [6]
    C.L. Oakley, G.H. Warrack and P.H. Clarke, J. Gen. Microbiol. 1, 1, 91 (1941).Google Scholar

Copyright information

© Consultants Bureau 1958

Authors and Affiliations

  • A. E. Gurvich
    • 1
    • 2
  • M. V. Ispolatovskaia
    • 1
    • 2
  1. 1.Laboratory of Physiological Chemistry Institute of Biological and Medical ChemistryAcad. Med. Sci. USSRMoscow
  2. 2.Department of Biochemistry Gamalela Institute of Epidemiology and MicrobiologyAcad. Med. Sci. USSRMoscow

Personalised recommendations