Advertisement

Molecular Biology Reports

, Volume 5, Issue 4, pp 251–252 | Cite as

Glutamate dehydrogenase activity in subcellular fractions of mouse fibrosarcoma

  • Marian Hillar
  • Billy Lennox
Article
  • 31 Downloads

Abstract

Glutamate dehydrogenase activity was found to be present at a high level in neoplastic cytosol and microsomes, 3.45 and 9.90 nmoles NADH/min mg protein, respectively. This remains in accordance with the high rate of RNA and protein synthesis in the neoplastic process.

Keywords

Glutamate Protein Synthesis Cytosol Dehydrogenase Activity Subcellular Fraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Hillar, M. “Regulation of glutamate dehydrogenase activity in isolated rat liver mitochondria by ADP and progesterone”, Biokhimiya 38, 548–551 (1973)Google Scholar
  2. 2.
    Hillar, M. “Glutamate dehydrogenase”, J. Bioenergetics 6, 89–126 (1974).Google Scholar
  3. 3.
    Hillar, M., Davis S.A. and Chambers, D., “Studies on subcellular poly(A)-mRNA-rich RNA fraction with glutamate dehydrogenase template activity”, Physiol. Chem. Phys. 9, 205–217 (1977).Google Scholar
  4. 4.
    Hillar, M., Davis, S.A., Nikbakht, K.N. and Chambers, D., “Translation of mRNA for glutamate dehydrogenase and spectrophotometric procedure to follow the enzyme biosynthesis”, Arch. Internl. Physiol. Biochim. 85, 538–588 (1977).Google Scholar
  5. 5.
    Hillar, M. and Reuben, J., “Hormonal induction of glutamate dehydrogenase in rat liver”, Experientia 32, 653–655 (1976).Google Scholar
  6. 6.
    Lowry, O.H., Rosenbrough, N.J., Farr, A.L. and Randall, R., “Protein measurement with the Folin phenol reagent”, J. Biol. Chem. 193, 265–275 (1951).Google Scholar
  7. 7.
    Milas, L., Hunter, N., Basic, I., Mason, K., Grdina, D.J. and Wither, H.R. “Nonspecific immunotherapy of murine solid tumors with Corynebacterium granulosum”, J. Natl. Canc. Inst. 54, 895–902 (1975).Google Scholar
  8. 8.
    Weber, G., “Enzymatic strategy of the cancer cell”, in Cancer Enzymology (Eds. J. Schultz and F. Ahmad), Academic Press, New York 1976, pp. 63–88.Google Scholar
  9. 9.
    Weinhouse, S., Shatton, J.B. and Morris, H.P., “Isozyme composition, gene regulation and metabolism of experimental hepatomas”, in Cancer Enzymology (Eds. J. Schultz and F. Ahmad), Academic Press, New York 1976, pp. 41–61.Google Scholar

Copyright information

© Dr. W. Junk bv Publishers 1979

Authors and Affiliations

  • Marian Hillar
    • 1
  • Billy Lennox
    • 1
  1. 1.Department of BiologyTexas Southern UniversityHoustonUSA

Personalised recommendations