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A simple and fast electrophoretic method for elution of nucleic acids from gels

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Abstract

A very convenient electrophoretic procedure for DNA or RNA elution from agarose or polyacrylamide gels is described. The gel piece with nucleic acid to be eluted is contained in a dialysis bag filled with buffer and elution is carried out in a horizontal electrophoresis apparatus. The nucleic acid is recovered with a high yield and can be used, without prior treatment, in further enzymatic or chemical reactions. Results obtained with DNA are presented here.

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Authors and Affiliations

  1. Section de Biologie, Institut Curie, Paris, (France)

    Younes Chouikh & Pierre Yot

  2. Institut de Recherche en Biologie Moleculaire, Universite Paris VII, Paris, (France)

    Michel Volovitch

Authors
  1. Younes Chouikh
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  2. Michel Volovitch
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  3. Pierre Yot
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Chouikh, Y., Volovitch, M. & Yot, P. A simple and fast electrophoretic method for elution of nucleic acids from gels. Mol Biol Rep 5, 237–239 (1979). https://doi.org/10.1007/BF00782896

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  • DOI: https://doi.org/10.1007/BF00782896

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