Molecular Biology Reports

, Volume 5, Issue 4, pp 221–224 | Cite as

rRNA topography in ribosome. IV. The accessibility of the 5'-end region of 16S rRNA

  • E. A. Skripkin
  • A. M. Kopylov
  • A. A. Bogdanov
  • S. V. Vinogradov
  • Yu. A. Berlin
Article
  • 25 Downloads

Abstract

The accessibility of the 5'-end region of 16S rRNA (A8GAGUUUG15) inEscherichia coli ribosomes for complementary binding with the synthetic octanucleotide d(CAAACTCT) has been studied. Nonequilibrium gel-filtration was used to evaluate parameters of the binding of this oligonucleotide with free 16S rRNA, ribosomal subunits, and 70S ribosomes. A simple approach is presented to calculate the apparent association constants and the number of binding sites based upon the data obtained under those conditions. Free 16S rRNA, 30S subunits, and 70S ribosomes were found to form rather stable complexes with the octanucleotide, the association constants being similar in all three cases. These data strongly suggest the surface location of the 16S rRNA 5'-end inE. coli ribosomes.

Keywords

Simple Approach Stable Complex Surface Location Association Constant Ribosomal Subunit 

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Copyright information

© Dr. W. Junk bv Publishers 1979

Authors and Affiliations

  • E. A. Skripkin
    • 1
  • A. M. Kopylov
    • 1
  • A. A. Bogdanov
    • 1
  • S. V. Vinogradov
    • 2
  • Yu. A. Berlin
    • 2
  1. 1.A.N. Belozersky Laboratory of Bioorganic Chemistry and Molecular BiologyMoscow State UniversityMoscowUSSR
  2. 2.M.M. Shemyakin Institute of Bioorganic ChemistryAcademy of Sciences of the USSRMoscowUSSR

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