Journal of comparative physiology

, Volume 145, Issue 1, pp 29–35 | Cite as

Adrenergic regulation of chloride secretion across the opercular epithelium: The role of cyclic AMP

  • Susan A. Mendelsohn
  • Bruce D. Cherksey
  • Kevin J. Degnan


Activation of the β-adrenergic receptors of the opercular epithelium ofFundulus heteroclitus stimulates Cl secretion, while activation of the α-adrenergic receptors inhibits Cl secretion (Degnan and Zadunaisky, 1979). The possible involvement of adenosine 3′, 5′-monophosphate (cAMP) in these adrenergic responses was investigated. Isolated opercular epithelia incubated in Ringer, containing 10 mM theophylline, had cAMP levels ranging between 5.3 and 19.3 pmoles·mg protein−1 (mean=9.5±1.0 pmoles·mg protein−1). Activation of the β-receptors by 10−5 M isoproterenol increased the mean cAMP level 430% (P<0.001). Blockage of the β-receptors with propranolol greatly reduced the increase in cAMP in response to isoproterenol. Activation of the α-receptors by 10−5 M arterenol stimulated the mean cAMP level 270% (P<0.01). However, when the β-receptors were blocked with propranolol, arterenol had no effect on the cAMP level. The possible involvement of Ca++ in these adrenergic responses was investigated. Neither the stimulatory effect of isoproterenol, nor the inhibitory effect of arterenol on the Cl secretion were diminished in the absence of extracellular Ca++. The Ca++ ionophore, A23187, and the calmodulin inhibitor, trifluoperazine, had no effects on the Cl secretion. The Ca++-channel blocker, D600, had a significant inhibitory effect (P<0.005). Guanosine 3′,5′-monophosphate (cGMP) had no effect on the Cl secretion.

The results indicate that β-adrenergic stimulation of Cl secretion across the opercular epithelium is accompanied by an elevation in tissue cAMP levels. α-adrenergic inhibition of Cl secretion does not involve changes in the tissue cAMP. Neither of these responses appear to require Ca++.


Propranolol Theophylline Monophosphate Guanosine Isoproterenol 
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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Susan A. Mendelsohn
    • 1
    • 2
  • Bruce D. Cherksey
    • 1
    • 2
  • Kevin J. Degnan
    • 1
    • 2
  1. 1.Department of Physiology and BiophysicsNew York University Medical CenterNew YorkUSA
  2. 2.Mt. Desert Island Biological LaboratorySalsbury CoveUSA

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