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Bulletin of Experimental Biology and Medicine

, Volume 57, Issue 4, pp 472–474 | Cite as

A comparative immunochemical study of antisera to a tissue homogenate and a mixture of its nonprotein fractions

  • V. S. Gostev
  • A. K. Saakov
  • A. E. Azletskaya
  • A. A. Perelaznyi
  • N. A. Nazarenko
  • N. M. Mazina
  • A. N. Kulagin
  • Yu. V. Zykov
  • A. A. Nikitenko
  • N. I. Skachkov
Microbiology and Immunology

Summary

Homogenate and a number of fractions free from soluble protein (lipid, polysaccharide, DNP) were obtained from the resected tissue of a human stomach cancer. Donkeys and horses were immunized with the homogenate or a mixture of the fractions mentioned with Freund's adjuvant. Gamma-globulin preparations were prepared from the antisera; their serological activity was studied in the complement fixation reaction with the test antigens — lipid, polysaccharide, DNP, saline extract.

Immunization with the antigen of a simplified composition (fractions without soluble protein) led to the formation of antibodies to these fractions in a higher titer and with a less marked cytotoxicity than immunization with a more complicated tissue antigenic mixture.

Keywords

Lipid Polysaccharide Soluble Protein High Titer Tissue Homogenate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Consultants Bureau Enterprises, Inc. 1964

Authors and Affiliations

  • V. S. Gostev
    • 1
  • A. K. Saakov
    • 1
  • A. E. Azletskaya
    • 1
  • A. A. Perelaznyi
    • 1
  • N. A. Nazarenko
    • 1
  • N. M. Mazina
    • 1
  • A. N. Kulagin
    • 1
  • Yu. V. Zykov
    • 1
  • A. A. Nikitenko
    • 1
  • N. I. Skachkov
    • 1
  1. 1.Laboratory of ImmunochemistryInstitute of Experimental Biology of the AMN SSSRMoscow

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