A comparative immunochemical study of antisera to a tissue homogenate and a mixture of its nonprotein fractions
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Homogenate and a number of fractions free from soluble protein (lipid, polysaccharide, DNP) were obtained from the resected tissue of a human stomach cancer. Donkeys and horses were immunized with the homogenate or a mixture of the fractions mentioned with Freund's adjuvant. Gamma-globulin preparations were prepared from the antisera; their serological activity was studied in the complement fixation reaction with the test antigens — lipid, polysaccharide, DNP, saline extract.
Immunization with the antigen of a simplified composition (fractions without soluble protein) led to the formation of antibodies to these fractions in a higher titer and with a less marked cytotoxicity than immunization with a more complicated tissue antigenic mixture.
KeywordsLipid Polysaccharide Soluble Protein High Titer Tissue Homogenate
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