Summary
Amyloid enhancing factor (AEF) activity was tested in spleen homogenates from 52, 28, and 9-week-old untreated mice. Strong AEF activity was present in the supernatant of spleen homogenate from the 52-week-old mice. Only weak activity was observed in the supernatant from the 28-week-old mice, and none was seen in that from the 9-week-old mice. Crude spleen homogenates from the 52-week-old mice were subjected to Sephacryl S-300 gel filtration. AEF activity existed in the first gel filtration peak. These findings suggest that AEF increases in the spleens of aged mice and that AEF may be a substance of high molecular weight.
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Yokota, T., Ishihara, T., Kawano, H. et al. Amyloid enhancing factor (AEF) in the aging mouse. Vichows Archiv A Pathol Anat 414, 511–514 (1989). https://doi.org/10.1007/BF00781708
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DOI: https://doi.org/10.1007/BF00781708