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A comparative cytological analysis of tissue culture cells under normal conditions and exposed to the action of poliomyelitis virus

Communication I. The trend of the cytological changes in 4 varities of cells cultivated in normal conditions

  • Experimental Biology
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Summary

The authors studied the morphology of the following cells: renal cells ofMacacus rhesus, cardiac cells ofMacacus cinomolgus, and of different lines of malignant HeLa and Hep cells. All these cells were cultured on mica films in the liquid nutrient medium. Although the dynamics of the cytological changes of the cultures were similar, each one of them had certain growth peculiarities reflected in the curves of mitotic activity. In 122 experiments this activity in the renal cells was low. Evidently, the cellular reproduction here occurs by amitosis. Irregular mitoses are encountered in all four cultures as a manifestation, of intensive cellular reproduction. The growth dynamics and morphology of the renal culture vary depending on the cells originating directly from the body or from a subculture. The culture of a cinornolgus heart differs by a number of signs (dynamics of mitotic activity, the shape of the nuclei) from the cultures of malignant cells, with which it has certain morphological similarities.

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Authors and Affiliations

  1. Moscow Research Institute of Preparations against Poliomyelitis, Moscow, USSR

    S. Ya. Zalkind & L. G. Stepanova

Authors
  1. S. Ya. Zalkind
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  2. L. G. Stepanova
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Zalkind, S.Y., Stepanova, L.G. A comparative cytological analysis of tissue culture cells under normal conditions and exposed to the action of poliomyelitis virus. Bull Exp Biol Med 47, 765–770 (1959). https://doi.org/10.1007/BF00781239

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  • DOI: https://doi.org/10.1007/BF00781239

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