Electrophoretic analysis of the composition of the specific precipitate

  • Yu. S. Tatarinov
Microbiology and Immunology
  • 10 Downloads

Summary

An electrophoretic analysis of the dissolved precipitate and supernatant fluid was conducted in the equivalent zone. For microelectrophoresis on paper, a Flynn and De Mayo type of chamber as modified by A. E. Gurvich was used. The specific precipitate, thoroughly washed with saline solution, largely consists of the γ-globulins of the immune serum (about 85%) and proteins, the electrophoretic mobility of which approaches that of the β2 and β-globulins. Albumins adsorbed by the specific antigen-antibody complex are lost in washing the precipitate. A suggestion is made that about 15% of the precipitate consists of nonspecific proteins.

Keywords

Public Health Albumin Saline Solution Electrophoretic Mobility Electrophoretic Analysis 

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Copyright information

© Consultants Bureau Enterprises, Inc. 1960

Authors and Affiliations

  • Yu. S. Tatarinov
    • 1
  1. 1.Department of Biological Chemistry of the A. V. Lunacharskii Astrakhan Medical InstituteUSSR

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