Electrophoretic analysis of the composition of the specific precipitate

  • Yu. S. Tatarinov
Microbiology and Immunology


An electrophoretic analysis of the dissolved precipitate and supernatant fluid was conducted in the equivalent zone. For microelectrophoresis on paper, a Flynn and De Mayo type of chamber as modified by A. E. Gurvich was used. The specific precipitate, thoroughly washed with saline solution, largely consists of the γ-globulins of the immune serum (about 85%) and proteins, the electrophoretic mobility of which approaches that of the β2 and β-globulins. Albumins adsorbed by the specific antigen-antibody complex are lost in washing the precipitate. A suggestion is made that about 15% of the precipitate consists of nonspecific proteins.


Public Health Albumin Saline Solution Electrophoretic Mobility Electrophoretic Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. [1]
    V. S. Gostev and A. K. Saakov, Zhur. mikrobiol., epidemiol. i immunobiol.5, 62 (1953).Google Scholar
  2. [2]
    V. S. Gostev, O. A. Popovkina, and A. K. Saakov, Zhur. mikrobiol., epidemiol. i immunobiol.9, 39 (1953).Google Scholar
  3. [3]
    A. E. Gurvich, Lab. delo3, 3 (1955).Google Scholar
  4. [4]
    A. E. Gurvich, Biokhimiya20,5, 550 (1955).Google Scholar
  5. [5]
    A. E. Gurvich and R. B. Kapner, Lab. delo2 23 (1958).Google Scholar
  6. [6]
    P. Burthin, Bull. Sol. chim.9–10, 977 (1955).Google Scholar
  7. [7]
    J.C. Heff and E.L. Becker, J. Immunol.3, 826 (1954).Google Scholar
  8. [8]
    M. Heidelberger and F.E. Kendall, J. Exper. Med.59, 519 (1934); J. Exper. Med.62, 697 (1936); Exper. Med.65, 647 (1937).Google Scholar
  9. [9]
    E. Kabat and M. Heidelberger, J. Exper. Med.66, 229 (1937).Google Scholar
  10. [10]
    O.H. Lowry, N.J. Rosebrough and others, J. Biol. Chem.193, 265 (1951).Google Scholar

Copyright information

© Consultants Bureau Enterprises, Inc. 1960

Authors and Affiliations

  • Yu. S. Tatarinov
    • 1
  1. 1.Department of Biological Chemistry of the A. V. Lunacharskii Astrakhan Medical InstituteUSSR

Personalised recommendations