The use of C¹⁴O₂ canopy techniques for measuring carbon transfer through the plant-soil system

Summary

Methods for labelling growing plants by exposing them to C¹⁴O₂ under a cellulose acetate-butyrate canopy have been developed for laboratory and field use. The length of labelling ranged from 2 to 33 days and the C¹⁴O₂ content of the atmosphere was automatically controlled. This made it possible to measure carbon assimilation by the plants, transfer of photosynthates beneath ground and respiration of the roots.

In the laboratory, root respiration of wheat plants was measured by separating the above and beneath ground plant parts using a RTV rubber partition. Half to two thirds of the assimilated carbon was found above ground, 15 to 25 per cent in the roots and shoot bases below the partition and 17 to 25 per cent was lost by underground respiration. The variability of these proportions was related to the stage of maturity of the plants.

On native grassland, the relative above and beneath ground productivity was 50 per cent. The time required for the photosynthates to reach the roots at various depths ranged from 1 to 5 days and the amount of material deposited in the roots changed with time and soil moisture content. The use of tubes inserted at various depths beneath the canopy permitted sampling of soil air for C¹⁴ and CO₂ measurements. The soil C¹⁴O₂ flux indicated that root respiration during 8 days accounted for 24 per cent of the labelled carbon translocated to the roots after a two days labelling period.