Plant and Soil

, Volume 38, Issue 2, pp 307–314 | Cite as

Cellulase in red clover exudates

  • P. M. Bonish


Cellulase was detected in the medium when red clover (Trifolium pratense) seedlings were grown aseptically in flasks. The amount of cellulase found depended on the ionic composition and pH of the medium. Cellulase was found when plants were grown in distilled water. With added salts the amount of cellulase detected was negligible at pH 5.5 or less, but increased with increasing pH; less was released when seedlings were grown throughout in the presence of CaCl2. The enzyme may be extracytoplasmic, located on the root surface, and released by changes in the salt content and pH of the medium.

Enzyme preparations contained at least two components. At pH 3.5 and 20°C one was stable for at least 22 h, whereas the other was destroyed within 0.5 h. The reaction rate of enzyme preparations was almost constant from pH 5 to 7.


Enzyme Plant Physiology CaCl2 Cellulase Enzyme Preparation 
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Copyright information

© Martinus Nijhoff, The Hague 1973

Authors and Affiliations

  • P. M. Bonish
    • 1
  1. 1.Soil Microbiology DepartmentRothamsted Experimental StationHarpendenEngland

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