Molecular Biology Reports

, Volume 7, Issue 1–3, pp 47–51 | Cite as

Properties of a 110000 molecular weight protein in rat liver hnRNP particles

  • A. Schweiger
  • G. Kostka
Article

Abstract

Particles carrying heterogeneous nuclear RNA (30–40 S-particles) were isolated from rat liver nuclei and the particle proteins separated by sodium dodecylsulfate gel electrophoresis. Some properties of a 110000 molecular weight component (P 110/103) were studied in detail: (i) P 110/103 was labeled to a 4–5 times higher specific activity than the major particle proteins in the presence of [14C]-amino acidsin vivo. (ii) In nuclei incubated with [3H]- or [32P]-nicotinamide adenine dinucleotide P 110/103 was labeled presumably by ADP-ribosylation. (iii) A protein with the same molecular weight as P 110/103 and isolated from the nuclear extract by affinity chromatography was phosphorylated in vitro.

Keywords

Molecular Weight Adenine Sodium Dodecylsulfate Dinucleotide Affinity Chromatography 

Abbreviations

hnRNA

heterogeneous nuclear RNA

hnRNP and mRNP

ribonucleoproteins which contain hnRNA respectively mRNA

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References

  1. 1.
    CardelliJ. & PitotH. C., 1978. Cancer Res. 38: 2099–2102.Google Scholar
  2. 2.
    SundquistB., PerssonT. & LindbergU., 1977. Nucl. Acid Res. 4: 899–915.Google Scholar
  3. 3.
    Tasseron-de JongJ. G., BrouwerJ., RietveldK., ZoetemelkC. E. M. & BoschL., 1979. Eur. J. Biochem. 100: 271–283.Google Scholar
  4. 4.
    MaxwellE. S. & FischerM. S., 1979. Biochem. Biophys. Acta 562: 319–330.Google Scholar
  5. 5.
    ChisickM. C., BrennesselB. A. & BiswasD. K., 1979. Biochem. Biophys. Res. Comm. 91: 1109–1116.Google Scholar
  6. 6.
    SchweigerA. & KostkaG., 1980. Expt. Cell Res. 125: 211–219.Google Scholar
  7. 7.
    KostkaG. & SchweigerA., 1980. Molec. Biol. Rep. 6: 57–61.Google Scholar
  8. 8.
    WeberK. & OsbornM. 1969. J. Biol. Chem. 244: 4406–4412.Google Scholar
  9. 9.
    BonnerW. M. & LaskeyR. A., 1974. Eur. J. Biochem. 46: 83–88.Google Scholar
  10. 10.
    HilzH. & StoneP., 1976. Rev. Physiol. Biochem. Pharmacol. 76: 1–58.Google Scholar
  11. 11.
    HayaishiO. & UedaK., 1977. Ann. Rev. Biochem. 46: 95–116.Google Scholar
  12. 12.
    IshikawaK., KurodaC. & OgataK., 1969. Biochim. Biophys. Acta 179: 316–331.Google Scholar
  13. 13.
    DurbanE., RollD., BecknerG. & BuschH., 1980. Fed. Proc. 39: 1886.Google Scholar
  14. 14.
    ClevelandD. W., FischerS. G., KirschnerM. W. & LaemmliU. K., 1977. J. Biol. Chem. 252: 1102–1106.Google Scholar

Copyright information

© Dr W. Junk Publishers 1981

Authors and Affiliations

  • A. Schweiger
    • 1
  • G. Kostka
    • 1
  1. 1.Max-Planck-Institut für BiochemieMartinsried bei MünchenDeutschland

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