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Hypomethylation of repair patches in HeLa cells

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Abstract

In HeLa cells, under conditions where normal semiconservative synthesis is suppressed by hydroxyurea, the excision repair process after irradiation by UV results in a small amount of incorporation of nucleotides into nonreplicated DNA. By labelling the cytosine moieties of these repair patches, and measuring the ratio between cytosine and 5-methylcytosine, we have found that the level of methylation of cytosine in repair patches five hours after UV-irradiation of the cells is about half of that observed in normal semiconservatively synthesized DNA.

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Authors and Affiliations

  1. Laboratory of Cell and Molecular Biology, Institute of Experimental Medicine, CNR, Viale Regina Elena 324, 00161, Rome, Italy

    T. Eremenko, F. Morelli & P. Volpe

  2. Dept. of Biological Chemistry, University of Rome, Rome, Italy

    F. Palitti & E. P. Whitehead

  3. Biology Dpt., DG 12, Commission of the European Communities, Bruxelles, Belgium

    E. P. Whitehead

  4. Dept. of Organic and Biological Chemistry, University of Naples, Naples, Italy

    P. Volpe

Authors
  1. T. Eremenko
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  2. F. Palitti
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  3. F. Morelli
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  4. E. P. Whitehead
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  5. P. Volpe
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Eremenko, T., Palitti, F., Morelli, F. et al. Hypomethylation of repair patches in HeLa cells. Mol Biol Rep 10, 177–182 (1985). https://doi.org/10.1007/BF00778526

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  • DOI: https://doi.org/10.1007/BF00778526

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