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Phenylalanyl-tRNA synthetase fromE. coli MRE-600: Localization of the phenylalanine binding sites on the subunits by affinity reagents

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Abstract

The phenylalanine and the phenylalanyl-tRNAPhe binding sites on the subunits of phenylalanyl-tRNA synthetase fromE.coli MRE-600 were localized using p-azidoanilidate of [14C]phenylalanine and N-bromoacetyl[14C]phenylalanyl-tRNAPhe. The phenylalanine recognizing site was shown to be situated on the α subunit of the enzyme in close proximity to the contact region of the α and β subunits and the phenylalanyl-tRNAPhe recognizing site on the β subunit. Transfer of the aminoacyl moiety from the α subunit to the β subunit of the enzyme was assumed to take place in the process of catalysis of the aminoacylation reaction.

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References

  1. BartmannP., HankeT. & HollerE., 1975. J. Biol. Chem. 250: 7668–7674.

    Google Scholar 

  2. BartmannP., HankeT., Hammer-RaberB. & HollerE., 1974. Biochem. Biophys. Res. Comm. 60: 743–747.

    Google Scholar 

  3. BeznedelnaiaN. I., GorshkovaI. I., LavrikO. I. & KhodyrevaS. N., 1981. Bioorgan. Chem. (USSR) 7: 826–833.

    Google Scholar 

  4. FayatG., BlanquetS., DessenPh., BatelierG. & WallerJ.-P., 1974. Biochimie 56: 35–41.

    Google Scholar 

  5. FavreA., BalliniJ. P. & HollerE., 1979. Biochemistry 18: 2887–2895.

    Google Scholar 

  6. GorshkovaI. I., LavrikO. I., NevinskyG. A. & KhutorianskaiaL. Z., 1975. Mol. Biol. (USSR) 9: 509–515.

    Google Scholar 

  7. GünterCh. & HollerE., 1979. Biochemistry 18: 2028–2038.

    Google Scholar 

  8. HankeT., BartmannP., HenneckeH., KosakowskiH. M., JaenickeR., HollerE. & BöckA., 1974. Eur. J. Biochem. 43: 601–607.

    Google Scholar 

  9. HenneckeH., 1976. FEBS Lett. 72: 182–186.

    Google Scholar 

  10. HenneckeH. & BöckA., 1975. Eur. J. Biochem. 55: 431–437.

    Google Scholar 

  11. HenneckeH., WalterI., FranzH. & Hoppe-Seyler'sZ., 1977. Physiol. Chem. 358: 197–208.

    Google Scholar 

  12. Lavrik, O. I. & Khodyreva, S. N., Biochemistry (USSR) 44: 570–571.

  13. LavrikO. I., MoorN. A., NevinskyG. A. & KhodyrevaS. N., 1981. Bioorgan. Chem. (USSR) 7: 834–843.

    Google Scholar 

  14. SandahchievL. S., StephanovichL. E., StarostinaV. K. & ChuchaevV. M., 1967. Mol. Biol. (USSR) 1: 463–466.

    Google Scholar 

  15. StulbergM. P., 1967. J. Biol. Chem. 242: 1060–1064.

    Google Scholar 

  16. VlasovV. V., LavrikO. I., MamaevS. V., KhodyrevaS. N., ChijikovV. E. & ShvalyeA. Ph., 1980. Mol. Biol. (USSR) 14: 531–537.

    Google Scholar 

  17. WeberK. & OsbornM., 1969. J. Biol. Chem. 244: 4406–4412.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Organic Chemistry, 630090, Novosibirsk, USSR

    O. I. Lavrik, N. A. Moor & S. N. Khodyreva

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  1. O. I. Lavrik
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  2. N. A. Moor
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  3. S. N. Khodyreva
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Lavrik, O.I., Moor, N.A. & Khodyreva, S.N. Phenylalanyl-tRNA synthetase fromE. coli MRE-600: Localization of the phenylalanine binding sites on the subunits by affinity reagents. Mol Biol Rep 8, 123–126 (1982). https://doi.org/10.1007/BF00778515

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  • DOI: https://doi.org/10.1007/BF00778515

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