Molecular Biology Reports

, Volume 4, Issue 4, pp 247–252 | Cite as

Intracellular distribution of biotin-14COOH in rat liver

  • F. Petrelli
  • P. Moretti
  • M. Paparelli
Article

Abstract

Biotin clearance, its distribution in liver and liver fractions after intravenous administration of 5 μCi/100 g body weight (21.55 μg) of biotin-14COOH in normal and biotin-deficient rats are reported. In the biotin deficient animal there is a more rapid disappearance of the labeled biotin from the blood stream. Biotin-14COOH incorporation in the liver of the deficient rat is more rapid and larger than the incorporation in normal rat liver. Almost all the biotin recovered from liver homogenate is found in the mitochondria and in the pH 5.2 cytosol fraction; whereas in the microsomes only a very small amount is present. The intracellular distribution of biotin is in agreement with its known metabolic roles.

Keywords

Body Weight Cytosol Biotin Intravenous Administration Blood Stream 

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Copyright information

© Dr. W. Junk bv Publishers 1978

Authors and Affiliations

  • F. Petrelli
    • 1
  • P. Moretti
    • 1
  • M. Paparelli
    • 1
  1. 1.Institute of General PhysiologyUniversity of CamerinoItaly

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