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Study of ribosomal deoxyribonucleic acid of sea urchin

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Abstract

The structure of ribosomal DNA (rDNA) satellite of sea urchin (Lytechinus variegatus) sperm has been re-examined after purification by two widely used methods. Saturation hybridization experiments indicate that about 28–32% of the rDNA contain sequences complementary to rRNA. Results of hyperchromic spectral analysis reveal that the rDNA melts as two distinct but unequal components. The early transition presumably corresponds to the melting of most of the transcribed part and the late transition is suggestive of the melting of a G+C-rich segment of the rDNA, which may be a non-transcribed spacer.

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Abbreviations

FAT :

fraction of all A+T pairs denatured

FGC :

fraction of all G+C pairs denatured

SSC:

standard saline citrate

rDNA:

template for ribosomal RNA

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Author notes

  1. Nirmal Kumar Mishra

    Present address: Department of Zoology, Patna University, 800 005, Patna, India

Authors and Affiliations

  1. Department of Zoology, University of North Carolina, Chapel Hill, N.C., USA

    Nirmal Kumar Mishra

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  1. Nirmal Kumar Mishra
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Additional information

This work is a part of the author's Ph.D. thesis (U.N.C., Chapel Hill).

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Mishra, N.K. Study of ribosomal deoxyribonucleic acid of sea urchin. Mol Biol Rep 4, 241–246 (1979). https://doi.org/10.1007/BF00777562

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  • DOI: https://doi.org/10.1007/BF00777562

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