Abstract
During theHeLa S-phase, DNA was methylated, at 1-hr intervals in isolated nuclei and fractionated in Ag+−Cs2SO4 gradients providing a heavy GC-rich peak and a main light AT-rich peak. Both size and specific methylation of these peaks changed during the nDNA duplicative phase. Replication of the heavy GC-rich nDNA fraction, which contains genes for ribosomal RNA, occurred inearly S; in contrast, replication of the main AT-rich nDNA fraction was maximal inlate S. Concomitantly, specific methylation of the GC-rich nDNA was maximal in the first part of S, while that of the AT-rich nDNA was maximal in the second part of S. This suggested that genes are replicated and methylated with order during the S-phase.
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Eremenko, T., Granieri, A. & Volpe, P. Organization, replication and modification of the human genome: Temporal order of synthesis and methylation of two classes of HeLa nDNA separated in Ag+−Cs2SO4 gradients. Mol Biol Rep 4, 237–240 (1979). https://doi.org/10.1007/BF00777561
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DOI: https://doi.org/10.1007/BF00777561