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Differential synthesis of lens proteins in the presence of m7 G(5′)pppG and cleavage product m7 GMP in an embryonic chick lens cell lysate

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Abstract

The effect of m7 GMP release from m7 GMP-containing mRNA cap sequence m7 GpppG by the embryonic chick lens m7 GpppN-pyrophosphatase activity on the synthesis of lens proteins was examined in a newly developed homologous translation system derived from 15-day embryonic chick lenses. The synthesis of total lens polypeptides and delta-crystallin polypeptides, the major translation product, was inhibited 84% and 88%, respectively, by 0.5 mM m7 GpppG; m7 GMP (0.5 mM) inhibited total synthesis by 63% but was 33% less inhibitory toward delta-crystallin synthesis; GppG and GMP were not inhibitors. m7 GpppG inhibited met-tRNA supmetinff incorporation into 80S initiation complexes.

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Authors and Affiliations

  1. Department of Biochemistry, New York University Dental Center, 10010, New York, New York, USA

    Gene C. Lavers

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  1. Gene C. Lavers
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Lavers, G.C. Differential synthesis of lens proteins in the presence of m7 G(5′)pppG and cleavage product m7 GMP in an embryonic chick lens cell lysate. Mol Biol Rep 4, 233–236 (1979). https://doi.org/10.1007/BF00777560

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  • DOI: https://doi.org/10.1007/BF00777560

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