Abstract
Low molecular weight peptides from calf thymus cause a strong dose-dependent stabilization of the DNA. The strength of DNA-peptide interaction is pH-dependent and decreases rapidly above pH 6.5. Moreover the complete kinetics of DNA denaturation and renaturation demonstrates that the peptide fraction increases significantly the DNA renaturation mostly at low temperature, showing that the interaction DNA-thymic effector helps the recombination of complementary DNA segments. The DNA stabilization rate by the peptide fraction is comparable to that obtained by means of high concentration of histones or synthetic polycationic peptides. However, the lack of basic amino acids in the peptide structure is not in favor of strong electrostatic interactions and implies a specific binding of peptide to DNA. The possible correlation of the specific thymic peptides-DNA interaction with the stereochemical kinking scheme of DNA is discussed.
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Gianfranceschi, G.L., Amici, D. and Guglielmi, L. (1975) Biochim. Biophys. Acta 414, 9–19.
Gianfranceschi, G.L., Amici, D. and Guglielmi, L. (1976) Nature 262, 622–623.
Amici, D., Rossi, G.B., Cioe, L., Matarese, G.P., Dolei, A., Guglielmi, L. and Gianfranceschi, G.L. (1977) Proc. Natl. Acad. Sci. U.S. 74, 3869–3873.
Gianfranceschi, G.L., Guglielmi, L., Amici, D., Bossa, F., Barra, D. and Petruzzelli, R. (1977) Mol. Biol. Rep. 3, 429–436.
Gianfranceschi, G.L., Amici, D. and Guglielmi, L. (1976) Mol. Biol. Rep. 3, 55–64.
Litman, R.M. (1968). J. Biol. Chem. 243, 6222–6233.
Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951) J. Biol. Chem. 193, 265–275.
Gilmour, R.S. and Paul, J. (1973) Proc. Natl. Acad. Sci. U.S. 70, 3440–3442.
Azbel, M.Y. (1973) Biopolymers 12, 1591–1609.
Gruenwedel, D.W. (1974) Biochim. Biophys. Acta 340, 16–30.
Shiao, D.D.F. and Sturtevant, J.M. (1973) Biopolymers 12, 1829–1836.
Privalov, P.L., Ptitsyn, O.B. and Birshtein, T.M. (1969) Biopolymers 8, 559–571.
Rubin, R.L. and Moudrianakis, E.N. (1972) J. Mol. Biol. 67, 361–374.
Olins, D.E., Olins, A.L. and vonHippel, P.H. (1967) J. Mol. Biol. 24, 157–176.
Spencer, M. (1977) Nature 267, 579–580.
Crick, F.H.C. and Klug, A. (1975) Nature 255, 530–533.
Sobell, H.M., Tsai, C.C., Gilbert, S.G., Jain, S.C. and Sakore, T.D. (1976) Proc. Natl. Acad. Sci. U.S. 73, 3068–3072.
Paul, J. and Gilmour, R.S. (1975) in The Structure and Function of Chromatin (Ciba Found. Symp. n. 28), pp. 181–192, Elsevier-Excerpta Medica-North Holland, Amsterdam.
Defer, N., Kitzis, A., Kruh, J., Brahms, S. and Brahms, J. (1977) Nucleic Acid Res. 4, 2293–2306.
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Guglielmi, L., Gianfranceschi, G.L., Venanzi, F. et al. Specific thymic peptides-DNA interaction. Correlation with the possible stereochemical kinking scheme of DNA. Mol Biol Rep 4, 195–201 (1979). https://doi.org/10.1007/BF00777553
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DOI: https://doi.org/10.1007/BF00777553