Abstract
Hen erythrocyte chromatin was digested with staphylococcal nuclease and fractionated by electrophoresis in polyacrylamide gels. Instead of the three bands described for mouse carcinoma chromatin, four main discrete components (MN1, MN2, MN2E and MN3) were resolved in the mononucleosome fraction of erythrocyte chromatin. MN2 contained all five histones and a DNA fragment of 165–180 base pairs. MN2E comprised four nucleosomal histones plus histone H5 (but not H1) and a DNA fragment of 170–190 base pairs. The relatively nuclease resistant MN3 fraction of erythrocyte nucleosomes contained H1 but no H5 histone. A more accurate analysis of the MN2 fraction in mouse carcinoma nucleosomes revealed some additional microheterogeneity depending on the presence of two different subfractions of H1.
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References
HewishD. and BurgoyneL. (1973) Biochem. Biophys. Res. Communs., 52, 504–510.
OlinsA.L. and OlinsD.E. (1974) Science 183, 330–332.
KornbergR.D. (1974) Science 184, 868–871.
NollM. (1974) Nature 251, 249–252.
OudetP., Gross-BellardM. and ChambonP. (1975) Cell 4, 281–299.
VarshavskyA.J., BakayevV.V., GeorgievG.P. (1976) Nucleic Acids Res., 3, 477–492.
BakayevV.V., BakayevaT.G. and VarshavskyA.J. (1977) Cell II, 619–629.
ToddR.D., and GarrardW.T. (1977) J. Biol. Chem. 252, 4729–4738.
WhitlockJ.P. and SimpsonR.T. (1976) Biochemistry 15, 3307–3313.
NollM. (1976) Cell 8, 349–355.
ComptonJ.L., BellardM. and ChambonP. (1976) Proc. Natl. Acad. Sci. USA. 73, 4382–4386.
MorrisN.R. (1976) Cell 9, 627–632.
LohrD., CordenJ., TatchellK., KovacicR.T. and vanHoldeK.E. (1977) Proc. Natl. Acad. Sci. USA 74, 79–83.
WeintraubH. and vanLenteF. (1974) Proc. Natl. Acad. Sci. USA 71, 4249–4253.
LaemmliU.K. (1970) Nature 227, 680–686.
BhorjeeR. and PedersonT. (1973) Biochemistry 12, 2716–2721.
IlyinYu.V., VarshavskyA.J., MickelsaarU.N. and GeorgievG.P. (1971) Europ. J. Biochem. 22, 235–245.
GoodwinG.H., SandersC. and JohnsE.W., (1973) Europ. J. Biochem. 38, 14–19.
Bakayev, V.V., Bakayeva, T.G., Shmatchenko, V.V., Georgiev, G.P. Europ. J. Biochem. in press.
HnilicaL.S. (1964) Experientia 20, 13–14.
OlinsA.L., CarlsonR.D., WrightE.B. and OlinsD.E. (1976) Nucleic Acids Res. 3, 3271–3291.
BustinM. and ColeR.D. (1969) J. Biol. Chem. 244, 5286–5290.
SmerdonM.J. and IsenbergI. (1976) Biochemistry 15, 4233–4242.
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Bakayeva, T.G., Bakayev, V.V. Separation of nucleosomes containing histones H1 and H5. Mol Biol Rep 4, 185–189 (1978). https://doi.org/10.1007/BF00777522
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DOI: https://doi.org/10.1007/BF00777522