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Molecular Biology Reports

, Volume 4, Issue 3, pp 137–141 | Cite as

Comparative properties of rat liver chromatinin situ andin vitro at early stages after partial hepatectomy

  • Olga Yu. Sondore
  • Galina E. Fedoseeva
  • V. A. Kadyckov
  • A. V. Zelenin
Article

Abstract

The binding of acridine orange and ethidium bromide to rat liver chromatin increases by 30% one hour after partial hepatectomy, returns to the control level by the second hour and increases again by the sixth hour. The changes described were found in investigations carried out on whole cells, on isolated nuclei, and on chromatin preparationin vitro. Increased ligand binding disappears after the treatment of the one-hour chromatin with a 0.3 M NaCl solution, but such a treatment does not change the binding of ligands to chromatin obtained six hours after hepatectomy. The one-hour chromatin is characterized by elongation of distances between individual nucleosomes whereas the two-hour chromatin is the same as in control.

Keywords

Bromide Ethidium Ethidium Bromide Ligand Binding Control Level 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Dr. W. Junk Publishers 1978

Authors and Affiliations

  • Olga Yu. Sondore
    • 1
  • Galina E. Fedoseeva
    • 1
  • V. A. Kadyckov
    • 1
  • A. V. Zelenin
    • 1
  1. 1.Institute of Molecular BiologyUSSR Academy of SciencesMoscowUSSR

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