Molecular Biology Reports

, Volume 5, Issue 1–2, pp 87–90 | Cite as

Poly(A+)mRNA sequences in free mRNP and polysomes of mouse ascites cells

  • Terence Martin
  • Michael McMullen
  • Phillip Shaw


The poly(A+)RNA of the free mRNP of mouse Taper ascites cell contains a very reduced number of different mRNA sequences compared to the polysome poly(A+)RNA. By the technique of mRNA:cDNA hybridization we have determined that the free mRNP contains approximately 400 different mRNA sequences while the polysomes contain about 9000 different mRNAs. The free mRNP poly(A+)RNA sequences are present in two abundance classes, the abundant free mRNP class containing 15 different mRNA sequences and the less abundant free mRNP class containing 400 different mRNAs. The polysome poly(A+)RNA consists of three abundance classes of 25, 500 and 8500 different mRNA sequences.

Despite its intracellular location in RNP structures not directly involved in protein synthesis the poly(A+)RNA purified from the free RNP of these cells was a very effective template for protein synthesis in cell-free systems. Cell-free translation products of free mRNP and polysome poly(A+)RNAs were analyzed by two-dimensional gel electrophoresis. This analysis confirmed the hybridization result that the free mRNP poly(A+)RNA contained fewer sequences than polysomal poly(A+)RNA. The abundant free RNP-mRNA directed protein products were a subset of the polysome mRNA-directed protein products. The numbers of more abundant products of cell-free protein synthesis directed by the free RNP-mRNA and polysomal mRNA were in general agreement with the hybridization estimates of the number of sequences in the abundant classes of these two mRNA populations.


mRNA Sequence Translation Product Abundance Class Abundant Product Ascites Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Dr. W. Junk bv Publishers 1979

Authors and Affiliations

  • Terence Martin
    • 1
    • 2
  • Michael McMullen
    • 1
    • 2
  • Phillip Shaw
    • 1
    • 2
  1. 1.Department of BiologyUniversity of ChicagoChicagoU.S.A.
  2. 2.Committee on GeneticsUniversity of ChicagoChicagoU.S.A.

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