Abstract
A simple procedure for the isolation and preparative gel electrophoresis of snRNA and scRNA is described. These small RNA species were used to select DNA sequences from a human genomic library which are able to protect hybridized snRNA or scRNA against T1-ribonuclease attack. The snRNA clones obtained contain only sequences for one snRNA species and only one copy of the respective gene. In contrast, more than one 7S RNA gene is present within the scRNA clones.
Similar content being viewed by others
References
ZieveG. W., 1981. Cell 25: 296–297.
LinkG. & BeneckeB. J., 1980. Planta 150: 406–411.
LernerM. R., BoyleJ. A., MountS. M., WolinS. L. & SteitzJ. A., 1980. Nature 283: 220–224.
RogersJ. & WallR., 1980. Proc. Natl. Acad. Sci. USA 77: 1877–1879.
BeneckeB. J., Ben-Ze'evA. & PenmanS., 1980. J. Cell. Phys. 103: 247–254.
ReichelR. & BeneckeB. J., 1980. Nucl. Acids Res. 8: 225–234.
RoopD. R., KristoP., StumphW. E., TsaiM. J. & O'MalleyB. W., 1981. Cell 23: 671–680.
MurphyJ. T., BurgessR. R., DahlbergJ. E. & LundE., 1982. Cell 29: 265–274.
LenkR., RansomL., KaufmanY. & PenmanS., 1977. Cell 10: 67–78.
BentonW. D. & DavisR. W., 1977. Science 196: 180–182.
DenhardtD. T., 1966. Biochem. Biophys. Res. Comm. 23: 641–646.
DenisonR. A., VanArsdellS. W., BernsteinL. B. & WeinerA. M., 1981. Proc. Natl. Acad. Sci. USA 78: 810–814.
SquiresC., LeeF., BertrandK., SquiresC. L., BransonM. J. & YanofskyC., 1975. J. Mol. Biol. 103: 351–381.
SouthernE. M., 1975. J. Mol. Biol. 98: 503–518.
ZieveG. & PenmanS., 1981. J. Mol. Biol. 145: 501–523.
BeneckeB. J. & HeideB., 1981. In: H. G.Schweiger (ed.) Intl. Cell Biol. 1980–1981. Springer Verlag, Berlin-Heidelberg-New York. pp. 53–59.
WeinerA. M., 1980. Cell 22: 209–218.
UlluE., MurphyS. & MelliM., 1982. Cell 29: 195–202.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zöphel, D., Jansen, H.W., Reichel, R. et al. Isolation and characterization of snRNA and scRNA gene candidates from a human genomic library. Mol Biol Rep 9, 59–64 (1983). https://doi.org/10.1007/BF00777474
Issue Date:
DOI: https://doi.org/10.1007/BF00777474