Molecular Biology Reports

, Volume 10, Issue 2, pp 65–68 | Cite as

Permeability to inhibitors of protein synthesis in virus infected cells

  • C. Fernández-Puentes


Infection of HeLa cells with different viruses induces permeabilization of the cell membrane to protein toxins such as α-sarcin. This phenomenon occurs with HeLa, KB, BHK-21 and L929 cells and EMC, SFV, VSV and Polio virus and is dependent on the ability of the virus to infect the cells. Inhibitors of endocytosis and lysosomotropic agents do not affect this process. Cells become sealed to the toxin approximately four hours after the infection. Sulfhydryl reagents impair cellular permeabilization to α-sarcin.


Cell Membrane Protein Synthesis HeLa Cell Infected Cell Sulfhydryl 
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  1. 1.
    CondeF. P., Fernández-PuentesC., MonteroM. T. V. & VázquezD., 1978. FEMS Microbiology Letters 4: 349–355.Google Scholar
  2. 2.
    ContrerasA. & CarrascoL., 1979. J. Virol. 29: 114–122.Google Scholar
  3. 3.
    Fernández-PuentesC., 1983. Mol. Cell. Biochem. 50: 185–191.Google Scholar
  4. 4.
    Fernández-PuentesC. & CarrascoL., 1980. Cell 20: 769–778.Google Scholar
  5. 5.
    Fernández-PuentesC. & VázquezD., 1977. FEBS Letters 78: 143–146.Google Scholar
  6. 6.
    FitzGeraldD. J. P., PadmanabhanR., PastanI. & WillinghamM. C., 1983. Cell 32: 607–617.Google Scholar
  7. 7.
    FuchsP., GruberE., GitelmanJ. & KohnA., 1980. J. Cell. Physiol. 103: 271–278.Google Scholar
  8. 8.
    FuchsP. & KohnA., 1983. Current Topics in Microbiology and Immunology. Vol 102, p. 58–99. Springer-Verlag.Google Scholar
  9. 9.
    KohnA., 1979. Advances Virus Res. 24: 223–276.Google Scholar
  10. 10.
    MarshM. & HeleniusA., 1980. J. Mol. Biol. 142: 439–454.Google Scholar
  11. 11.
    UchidaT., IchikawaT., ShimauchiY., IshikuaT. & OzakiA., 1971. J. Antibiotics 24: 259–262.Google Scholar

Copyright information

© Dr W. Junk Publishers 1984

Authors and Affiliations

  • C. Fernández-Puentes
    • 1
  1. 1.Departamento de Microbiología, Centro de Biología MolecularUniversidad Autónoma de MadridMadridSpain

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