Molecular Biology Reports

, Volume 8, Issue 4, pp 213–216 | Cite as

Synthesis of full-length cDNAs from partially purified human procollagen mRNAs

  • A. P. Ryskov
  • M. I. Prosnyak
  • S. A. Korneev
  • S. A. Limborska
Article
  • 15 Downloads

Abstract

Partially purified human procollagen mRNAs have been copied using reverse transcriptase from avian myeloblastosis virus. Under standard conditions, using a high deoxynucleotide concentration, only incomplete cDNAs corresponding to one-fifth to one-half of the template could be synthesized. Addition of a ribonuclease inhibitor from human placenta in the reaction mixture allowed the synthesis of full-length cDNA copies from extremely long procollagen mRNA.

Keywords

Reverse Transcriptase Standard Condition Ribonuclease Inhibitor Human Placenta cDNA Copy 

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Copyright information

© Dr W. Junk Publishers 1982

Authors and Affiliations

  • A. P. Ryskov
    • 1
  • M. I. Prosnyak
    • 2
  • S. A. Korneev
    • 2
  • S. A. Limborska
    • 2
  1. 1.Institute of Molecular BiologyUSSR Academy of SciencesMoscowUSSR
  2. 2.Institute of Medical GeneticsUSSR Academy of Medical SciencesMoscowUSSR

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