Molecular Biology Reports

, Volume 8, Issue 4, pp 213–216 | Cite as

Synthesis of full-length cDNAs from partially purified human procollagen mRNAs

  • A. P. Ryskov
  • M. I. Prosnyak
  • S. A. Korneev
  • S. A. Limborska


Partially purified human procollagen mRNAs have been copied using reverse transcriptase from avian myeloblastosis virus. Under standard conditions, using a high deoxynucleotide concentration, only incomplete cDNAs corresponding to one-fifth to one-half of the template could be synthesized. Addition of a ribonuclease inhibitor from human placenta in the reaction mixture allowed the synthesis of full-length cDNA copies from extremely long procollagen mRNA.


Reverse Transcriptase Standard Condition Ribonuclease Inhibitor Human Placenta cDNA Copy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Dr W. Junk Publishers 1982

Authors and Affiliations

  • A. P. Ryskov
    • 1
  • M. I. Prosnyak
    • 2
  • S. A. Korneev
    • 2
  • S. A. Limborska
    • 2
  1. 1.Institute of Molecular BiologyUSSR Academy of SciencesMoscowUSSR
  2. 2.Institute of Medical GeneticsUSSR Academy of Medical SciencesMoscowUSSR

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