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Distribution of chromatin proteins between fractions of hamster liver chromatin differing in their susceptibility to micrococcal nuclease

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Abstract

Hamster liver nuclei were fractionated by digestion with micrococcal nuclease into nuclease released (SP) and nuclease resistant (PP) fractions varying in chemical composition and transcriptional activity. Electrophoretic analysis of histones from SP and PP showed no qualitative and quantitative differences. Apparently chromatin-bound protease activity can be found in both fractions. Nonhistone chromatin proteins isolated from SP and PP under mild conditions were fractionated by hydroxyapatite chromatography into NHCP1, NHCP2, NHCP3 and molecular heterogeneity and specificity were tested by SDS-polyacrylamide gel electrophoresis.

The differences observed in nonhistone proteins are mainly of quantitative nature, however some specific polypeptides for SP and PP are observed.

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References

  1. Albrecht, J. & Hemminki, K., 1977. Biochim. Biophys. Acta 475: 152–159.

    Google Scholar 

  2. Arnold, E. A. & Young, K. E., 1974. Arch. Biochem. Biophys. 164: 73–89.

    Google Scholar 

  3. Arnold, E. A. & Young, K. E., 1976. Nucleic Acid Res. 3: 299–314.

    Google Scholar 

  4. Aström, S. & Von der Decken, A., 1980. Life Sciences 26: 797–804.

    Google Scholar 

  5. Bloom, K. S. & Anderson, J. N., 1978. Cell 15: 141–150.

    Google Scholar 

  6. Bokhon'ko, A. & Reeder, R. H., 1976. Biochem. Biophys. Res. Commun. 70: 146–152.

    Google Scholar 

  7. Briggs, R. C., Rothstein, H. & Wainwright, N., 1976. Expl. Cell Res. 99: 95–105.

    Google Scholar 

  8. Burton, K., 1956. Biochem. J. 62: 315–322.

    Google Scholar 

  9. Chauveau, J., Moule, Y. & Rouiller, C., 1956. Expl. Cell Res. 11: 317–321.

    Google Scholar 

  10. Chiu, J.-F. & Hnilica, L. S., 1977. In: Li, H. J. & Eckhardt, R. (eds.), Chromatin and Chromosome Structure. Academic Press, New York, pp. 193–254.

    Google Scholar 

  11. Crepin, M. & Dastuque, B., 1979. Eur. J. Biochem. 99: 499–505.

    Google Scholar 

  12. Dimitriadis, G. J. & Tata, J. R., 1980. Biochem. J. 187: 467–477.

    Google Scholar 

  13. Djondjurov, L., Ivanova, E. & Tsanev, R., 1979. Eur. J. Biochem. 97: 133–139.

    Google Scholar 

  14. Felsenfeld, G., 1978. Nature 271: 115–122.

    Google Scholar 

  15. Finch, J. T. & Klug, A., 1976. Proc. Natl. Acad. Sci. USA 73: 1897–1901.

    Google Scholar 

  16. Garel, A. & Axel, R., 1976. Proc. Natl. Acad. Sci. USA 73: 3966–3970.

    Google Scholar 

  17. Gates, D. M. & Bekhor, I., 1980. Science 207: 661–662.

    Google Scholar 

  18. Georgieva, E. J., Pashev, I. G. & Tsanev, R. G., 1981. Biochem. Biophys. Acta 652: 240–244.

    Google Scholar 

  19. Goodwin, G. H., Woodhead, L. & Johns, E. W., 1977. Febs Lett. 73: 85–88.

    Google Scholar 

  20. Gottesfeld, J. M., Garrard, W. M., Bagi, G., Wilson, R. F. & Bonner, J., 1974. Proc. Natl. Acad. Sci. USA 71: 2193–2197.

    Google Scholar 

  21. Gottesfeld, J. M., 1980. Nucleic Acids Res. 8: 905–922.

    Google Scholar 

  22. Kiliańska, Z., Sklodowska, M. & Klyszejko-Stefanowicz, L., 1980. Acta Biochim. Polon. 27: 89–97.

    Google Scholar 

  23. Kornberg, R., 1974. Science 184: 868–871.

    Google Scholar 

  24. Kuehl, L., Lyness, T., Dixon, G. H. & Levy-Wilson, B., 1980. J. Biol. Chem. 255: 1090–1095.

    Google Scholar 

  25. Lacy, E. & Axel, R., 1975. Proc. Natl. Acad. Sci. USA 72: 3978–3982.

    Google Scholar 

  26. Laemmli, U. K., 1970. Nature 227: 680–685.

    Google Scholar 

  27. Lau, A. F. & Rudden, R. W., 1977. Exptl. Cell Res. 107: 35–46.

    Google Scholar 

  28. Levy, B. & Dixon, G. H., 1978. Nucleic Acids Res. 5: 4155–4163.

    Google Scholar 

  29. Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J., 1951. J. Biol. Chem. 193: 265–275.

    Google Scholar 

  30. Mardian, J. K. W., Paton, A. E., Bunick, G. J. & Olins, D. E., 1980. Science 209: 1534–1536.

    Google Scholar 

  31. Panyim, S. & Chalkley, R., 1969. Arch. Biochem. Biophys. 130: 337–346.

    Google Scholar 

  32. Paul, I. J. & Duerksen, J. D., 1977. Can. J. Biochem. 55: 1140–1144.

    Google Scholar 

  33. Pederson, T. & Bhorjee, J. S., 1975. Biochemistry 14: 3238–3242.

    Google Scholar 

  34. Schneider, W. C., 1957. In: Colowick, S. P. & Caplan, N. O. (eds.), Methods in Enzymology. Academic Press, New York, V3, pp. 680–684.

    Google Scholar 

  35. Spelsberg, T. C. & Hnilica, L. S., 1971. Biochim. Biophys. Acta 228: 202–211.

    Google Scholar 

  36. Weber, K. & Osborn, M., 1969. J. Biol. Chem. 244: 4406–4411.

    Google Scholar 

  37. Weintraub, H. & Groudine, M., 1976. Science 193: 848–856.

    Google Scholar 

  38. Weisbrod, S. & Weintraub, H., 1979. Proc. Natl. Acad. Sci. USA 76: 630–634.

    Google Scholar 

  39. Weisbrod, S. & Weintraub, H., 1981. Cell 23: 391–400.

    Google Scholar 

  40. Zweidler, A., 1978. In Stein, G. Stein, J. & Kleinsmith L. J. (eds.), Methods in Cell Biology. Academic Press, New York V. 17, pp. 223–232.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, University of Lódź, 90-237, Lódź, Poland

    Z. Kiliańska, A. Lipińska, W. M. Krajewska & L. Klyszejko-Stefanowicz

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  1. Z. Kiliańska
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  2. A. Lipińska
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  3. W. M. Krajewska
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  4. L. Klyszejko-Stefanowicz
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Kiliańska, Z., Lipińska, A., Krajewska, W.M. et al. Distribution of chromatin proteins between fractions of hamster liver chromatin differing in their susceptibility to micrococcal nuclease. Mol Biol Rep 8, 203–211 (1982). https://doi.org/10.1007/BF00776581

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  • DOI: https://doi.org/10.1007/BF00776581

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