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Detection of nucleoside triphosphate binding sites of two types in escherichia coli RNA-polymerase by affinity labeling

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Abstract

The affinity labeling of E. coli RNA polymerase by periodate-oxidized uridin triphosphate (o-UTP) has been carried out under the conditions of poly(dA) and poly(dT) transcription. The extent of RNA polymerase labeling proved to be 2.5 times higher under the transcription of poly(dA) as compared to poly(dT). The amount of o-UTP attached to ββ'-subunits has been found to decrease if RNA polymerase is labeled in the transcribed complex with poly(dT). These results as well as those obtained in our previous study (1), suggest that there are two types of binding sites for nucleoside triphosphates and their analogs in E. coli RNA polymerase.

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References

  1. Slepneva, I.A., Zakharova, N.V., Backer, J.M. FEBS Lett. 87, 273–276 (1978).

    Google Scholar 

  2. Burgess, R.R., Jendrisak, J.J. Biochemistry 14, 4634–4638 (1975).

    Google Scholar 

  3. Burgess, R.R., J. Biol. Chem. 244, 6160–6167 (1969).

    Google Scholar 

  4. Richardson, J. Proc. Nat. Acad. Sci. U.S.A. 55, 1616–1623 (1966).

    Google Scholar 

  5. Lehman, I.R., Bessman, M.J., Simms, E.S., Kornberg, A. J. Biol. Chem. 233, 163–170 (1958).

    Google Scholar 

  6. Krakow, J.S., Goolsby, S.P. Biochem. Biophys. Res. Comm. 44, 453–458 (1971).

    Google Scholar 

  7. Powell, J.T., Brew, K., Biochemistry 15, 3499–3505 (1976).

    Google Scholar 

  8. Easterbrook-Smith, S.B., Walleace, J.C., Keech, D.B. Eur. J. Biochem. 62, 125–130 (1976).

    Google Scholar 

  9. Nixon, J., Spoor, T., Evans, J., Kimball, A. Biochemistry 11, 4570–4573 (1972).

    Google Scholar 

  10. Wu, F.Y.-H., Wu, C.-W. Biochemistry 13, 2562–2566 (1974).

    Google Scholar 

  11. Malcolm, A.D., Moffatt, J.R., Biochem. J. 175, 189–192 (1978).

    Google Scholar 

  12. Speckhard, D.C., Wu, F.Y.-H., Wu, C.-W. Biochemistry 16, 5228–5234 (1977).

    Google Scholar 

  13. Zillig, W., Palm, P., Heil, A. in RNA polymerase, Cold Spring Harbor Laboratory, p. 101 (1976).

  14. Sverdlov, E.D., Zarev, S.A., Modjanov, N.N., Grachev, M.A., Zaychikov, E.F., Pletnev, A.G. Bioorgan. Khimiya 4, 1278–1280 (1978).

    Google Scholar 

  15. Travaglini, E.C., Mildvan, A.S., Loeb, L.A. J. Biol. Chem. 250, 8647–8656 (1975).

    Google Scholar 

  16. Buttula, N., Dube, D.K., Loeb, L.A. J. Biol. Chem. 250, 8404–8408 (1975).

    Google Scholar 

Download references

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Authors and Affiliations

  1. Institute of Chemical Kinetics and Combustion, 630090, Novosibirsk, USSR

    I. A. Slepneva

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  1. I. A. Slepneva
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Slepneva, I.A. Detection of nucleoside triphosphate binding sites of two types in escherichia coli RNA-polymerase by affinity labeling. Mol Biol Rep 6, 31–34 (1980). https://doi.org/10.1007/BF00775751

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  • DOI: https://doi.org/10.1007/BF00775751

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