Abstract
A procedure was developed for the isolation of low molecular weight peptides (deprimerones) from calf thymus nuclei and other tissues. These peptides are active in controlling transcription and translation in cell-free systems, and stabilize the double stranded structure of DNA. The procedure involves extaction of nuclei with 80% ethanol at pH 9.5 and fractionation of the extracted peptides on Sephadex G-25. The isolated deprimerones represent heterogenous peptidic fractions which can be separated into four activity peaks on Sephadex G-25 column and into ten peptidic spots on two-dimensional cellulose gel thin layer chromatography. One of the peaks is identical to a chromatin peptidic fraction isolated previously by binding to DNA-cellulose. The deprimerones contain about 8 or 9 different amino acids. It was demonstrated that the cytoplasm contains about 10% of the deprimerones present in nuclei. They are also ubiquitous, since they were found in each of the tissues studied: calf thymus, mouse thymus, mouse spleen and mouse liver.
Similar content being viewed by others
References
HillarM. and PrzyjemskiJ., Biochim. Biophys. Acta 564, 246–263 (1979).
Hillar, M. and Przyjemski, J., Mol. Biol. Reports (1980) (in press).
GianfranceschiG., HillarM., ChanJ.T. and AmiciD., Mol. Biol. Reports 6, 27–29 (1980).
Hillar, M., Przyjemski, J. and Stolzmann, Z., Mol. Biol. Reports (1980) (in press).
AmiciD., RossiG.B., CioeL., MatareseG.P., DoleiA., GuglielmiL. and GianfranceschiG.L., Proc. Natl. Acad. Sci. U.S.A. 74, 3869–3873 (1977).
GianfranceschiG.L., GuglielmiL., AmiciD., BossaF., BarraD. and PetruzzelliR., Mol. Biol. Reports 3, 429–436 (1977).
PelhamH.R.B. and JacksonR.J., Eur. J. Biochem. 67, 247–256 (1976).
LowryO.H., RosebroughN.J., FarrA.L. and RandallR.J., J. Biol. Chem. 193, 265–275 (1951).
SeilerN., in: Methods in Biochemical Analysis (GlickD., ed.) Vol. 18, pp. 259–337, Interscience Publishers, New York, 1970.
HillarM., RangayyaV., JafarB.B., ChambersD., VitzuM. and WybornyL.E., Arch. Internatl. Physiol. Biochim. 87, 29–49 (1979).
MejbaumW., Hoppe Seyler's Z. Physiol. Chem. 258, 117–120 (1939).
Dische, Z., The Nucleic Acids (Chargaff, E. and Davidson, J.N., eds.) Vol. 1, pp. 285–305 (1955).
Lindberg, O. and Ernster, L. in: Methods of Biochemical Analysis (Glick, D., ed.) Vol. 3, pp. 1–19 (1956).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hillar, M., Przyjemski, J. & Wyborny, L.E. Studies on nuclear deprimerones: Isolation, fractionation, characterization and control of gene expression. Mol Biol Rep 6, 169–177 (1980). https://doi.org/10.1007/BF00775412
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00775412