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Phospholipid composition of human erythrocyte spectrin

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Abstract

Human erythrocyte spectrin, which has been extracted at low ionic strength and precipitated at pH 5.1, contains 0.025 μmoles phospholipid/mg protein. The composition of the spectrin-phospholipids differs from that of the erythrocyte membrane. A remarkable similarity was found between the composition of the “available” phospholipids of the inner leaflet of the membrane bilayer and the spectrinassociated phospholipids. Rebinding studies with1 2 5I-labeled spectrin show that the labeled spectrin binds preferentially to the cytoplasmic side of the membrane and that this binding is influenced by protein perturbations.

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Authors and Affiliations

  1. Fachbereich Biologie, Universität Konstanz, D-775, Konstanz, GFR

    E. Weidekamm, B. Brdiczka & M. Wildermuth

Authors
  1. E. Weidekamm
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  2. B. Brdiczka
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  3. M. Wildermuth
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Weidekamm, E., Brdiczka, B. & Wildermuth, M. Phospholipid composition of human erythrocyte spectrin. Mol Biol Rep 4, 25–28 (1978). https://doi.org/10.1007/BF00775176

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  • DOI: https://doi.org/10.1007/BF00775176

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