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Localization of host poly(A)+ mRNA in the ribosome profile of mengovirus-infected Ehrlich ascites tumor cells

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Abstract

The distribution of poly(A)+ mRNA among polysomes, monosomes, and ribosome-free supernatant fractions after mengovirus infection of Ehrlich ascites tumor (EAT) cells was investigated employing sucrose gradient centrifugation of their corresponding postnuclear supernatants. Poly(A)+ mRNA was isolated from sucrose gradient fractions and quantitated in a cell-free protein synthesizing system from uninfected EAT cells. It was also localized by annealing [3H]-poly(U) to the poly(A)-tracts of mRNA present in the sucrose gradient fractions. Both experiments revealed a gradual shift of host poly(A)+ mRNA from large to small polysomes and monosomes, respectively, with the time postinfection. The greatest part of host template RNA appears to remain ribosome-bound and only a fraction seems to be detached from the ribosomes in the course of mengovirus infection. At the end of the infectious cycle, 8 h postinfection, approximately 70% of the poly(A)+ mRNA detected in uninfected cells is still biologically active, but not translated in vivo, in agreement with data from the [3H] poly(U) hybridization experiment.

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Authors and Affiliations

  1. Max-Planck-Institut für Zellbiologie, 2940, Wilhelmshaven, Federal Republic of Germany

    I. Buchwald, M. Bialdiga, U. Traub & P. Traub

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  1. I. Buchwald
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  2. M. Bialdiga
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  3. U. Traub
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  4. P. Traub
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Buchwald, I., Bialdiga, M., Traub, U. et al. Localization of host poly(A)+ mRNA in the ribosome profile of mengovirus-infected Ehrlich ascites tumor cells. Mol Biol Rep 4, 9–13 (1978). https://doi.org/10.1007/BF00775173

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  • DOI: https://doi.org/10.1007/BF00775173

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