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Molecular Biology Reports

, Volume 10, Issue 1, pp 57–62 | Cite as

Murine splenocytes release an inhibitory peptide of lymphocyte DNA synthesis into serum-free culture medium

Article

Abstract

Murine splenocytes release a novel repressing activity of Concanavalin A-induced lymphocyte DNA synthesis. This activity was purified by gel filtration, ion exchange and thin layer chromatography. It was characterized to be a heat-stable peptide whose molecular weight was estimated to be about 1 000 daltons. The strong inhibition by this peptide was observed when added at early periods of the cell culture. This factor does not affect early RNA synthesis in lymphocytes, but nucleotide (UTP) incorporation at early periods was considerably decreased. In addition, although the factor inhibits drastically lymphocyte DNA synthesis, the cell viability is not changed significantly. These results suggest that the peptide suppresses the cell membrane function at early periods and specifically blocks the concomitant DNA synthesis.

Keywords

Peptide Nucleotide Thin Layer Chromatography Early Period Strong Inhibition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Dr. W. Junk Publishers 1984

Authors and Affiliations

  • Y. Okai
    • 1
  1. 1.Department of ImmunologyOEH University Medical SchoolKitakyushuJapan

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