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Molecular Biology Reports

, Volume 10, Issue 1, pp 41–47 | Cite as

Psoriatic hair follicle cells I. Biochemistry and behaviour in culture

  • A. J. M. Vermorken
  • M. C. Lenoir
  • B. Shroot
  • P. M. Kimenai
  • A. M. G. Vermeesch-Markslag
Article

Abstract

It is generally accepted that in psoriasis there is an alteration of epidermal cell proliferation. It has been reported that an increased rate of thymidine incorporation into keratinocytes is found in the upper part of the hair follicle in involved skin, but this is not the case in the lower part. Here we show that cells from psoriatic hair follicles could be brought in culture under the same conditions as those of normal hair follicles. Cells, whether originating from the upper or lower part of the hair follicle sheath either from involved or uninvolved psoriatic skin, show a faster rate of outgrowth in the first days of culture. Moreover, a large number of psoriatic cells have an increased motility in the early stages of culture, as compared to control cells. These properties can no longer be observed after several days in culture. The activity of glucose-6-phosphate dehydrogenase known to be increased in psoriatic plaques is normal in hair follicles isolated from these plaques. Protein gel electrophoretic investigations showed that there is no difference in gel patterns between normal and psoriatic hair follicles.

In conclusion, the isolation of human hair follicles represents a simple method that allows psoriatic keratinocytes to be brought in culture and permits the study of certain aspects of the disease.

Keywords

Psoriasis Hair Follicle Thymidine Incorporation Human Hair Follicle Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    FisherL. B. & WellsG. L., 1968. British Journal of Dermatology 80: 235–240.Google Scholar
  2. 2.
    FuchsE. & GreenH., 1980. Cell 19: 1033–1042.Google Scholar
  3. 3.
    FuchsE. & GreenH., 1981. Cell 25: 617–625.Google Scholar
  4. 4.
    HalprinK. M. & OhkawaraA., 1966. Journal of Investigative Dermatology 46: 51–69.Google Scholar
  5. 5.
    HukkelhovenM. W. A. C., VermorkenA. J. M. & BloemendalH., 1980. Preparative Biochemistry 10: 473–481.Google Scholar
  6. 6.
    KruegerG. G., 1981. In: R. L.Dobson & H.Thiers (eds.) Yearbook of Dermatology. Yearbook Medical Publishers, Chicago-London, pp. 13–70.Google Scholar
  7. 7.
    LaemmliU. K., 1970. Nature 227: 680–685.Google Scholar
  8. 8.
    MiguelA., RamonM., PetitpierreE., GoosC. M. A. A., Vermeesch-MarkslagA. M. G. & VermorkenA. J. M., 1983. Human Genetics 64: 576–579.Google Scholar
  9. 9.
    ShahradP. & MarksR., 1976. British Journal of Dermatology 94: 7–12.Google Scholar
  10. 10.
    VermorkenA. J. M., WeteringsP. J. J. M. & BloemendalH., 1978. Molecular Biology Reports 4: 211–216.Google Scholar
  11. 11.
    VermorkenA. J. M., WirtzP., SpierenburgG. Th., VanBennekomC. A., DeBruynC. H. M. M. & OeiT. L., 1979. Journal of Clinical Chemistry and Clinical Biochemistry 17: 325–329.Google Scholar
  12. 12.
    WeberK. & OsbornM., 1969. Journal of Biological Chemistry 244: 4406–4412.Google Scholar
  13. 13.
    WeteringsP. J. J. M., VermorkenA. J. M. & BloemendalH. 1981. British Journal of Dermatology 104: 1–5.Google Scholar
  14. 14.
    WeteringsP. J. J. M., VermorkenA. J. M. & BloemendalH., 1982. Experimental Cell Research 139: 439–443.Google Scholar
  15. 15.
    WyattE. H. & RiggottJ. M., 1981. British Journal of Dermatology 105: 349–350.Google Scholar

Copyright information

© Dr. W. Junk Publishers 1984

Authors and Affiliations

  • A. J. M. Vermorken
    • 1
    • 2
  • M. C. Lenoir
    • 1
  • B. Shroot
    • 1
  • P. M. Kimenai
    • 1
    • 2
  • A. M. G. Vermeesch-Markslag
    • 1
  1. 1.Department of BiochemistryCentre International de Recherches Dermatologiques (CIRD)ValbonneFrance
  2. 2.Research Unit for Cellular Differentiation and TransformationUniversity of NijmegenNijmegenThe Netherlands

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