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Effects of ozone on some biological activities of cellsin vitro

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Abstract

The aim of this work was to study thein vitro effect of ozone on the 70 kDa family of inducible heat shock proteins (HSPs 70). We also performed tests to investigate possible toxic effects of ozone at the different doses employed. In human haematic mononucleated cells ozone at doses up to 20 μg/ml had no toxic effects and induced biosynthesis of the HSPs70. Biosynthesis of these proteins was greater at 40 μg/ml. In murine macrophages testing with tetrazolium salt (MTT), neutral red, and 2-deoxy-d-[1-3H]glucose uptake and study of the cell morphology showed a remarkable resistance or no toxic effects at a dose of 100 μg/ml also. Melanoma B16 murine cells assayed with the MTT test demonstrated less resistance to the toxic effects of ozone than normal cells. These results provide indications relevant to the problems of ozone therapy.

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Abbreviations

ConA:

concanavalin A

HHMC:

human haematic mononucleated cells

HSPs70:

70 kDa family heat shock proteins

LPS:

lipopolysaccharide

MTT:

(3-(4,5-dimethylthiazol-2-yl)-diphenyltetrazolium bromide)

NR:

neutral red

PHA:

phytohaemagglutinin

TPA:

1,2-O-tetradecanoylphorbol 1,3-acetate

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Authors and Affiliations

  1. Institute of Human Physiology, University of Catania, Viale Andrea Doria 6, 95125, Catania, Italy

    V. Cardile, X. Jiang, F. Casella & M. Bindoni

  2. Institute of Biochemistry, University of Catania, Viale Andrea Doria 6, 95125, Catania, Italy

    A. Russo & M. Renis

Authors
  1. V. Cardile
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  2. X. Jiang
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  3. A. Russo
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  4. F. Casella
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  5. M. Renis
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  6. M. Bindoni
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Cardile, V., Jiang, X., Russo, A. et al. Effects of ozone on some biological activities of cellsin vitro . Cell Biol Toxicol 11, 11–21 (1995). https://doi.org/10.1007/BF00769988

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  • DOI: https://doi.org/10.1007/BF00769988

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