Abstract
The effect of the supernatants obtained from the liquid culture medium ofSporothrix schenkii andCandida albicans on the generation of superoxide anion (O −2 and hydroxyl radicals OH., the elements of reactive oxygen species (ROS), and chemilimunescence (CL), a measure of several ROS, by polymorphonuclear leukocytes (PMNs) was examined. In our study, it was shown that the supernatant ofS. schenkii increased all types of ROS generation examined and CL, while that ofC. albicans increased OH. generation and CL. The effect of the supernatants ofS. schenkii on OH. generation and CL and that ofC. albicans on CL were most remarkable when the supernatant obtained 8 weeks after the inoculation was used. The supernatant ofS. schenkii was shown to be a much more potent stimulant than the supernatant ofC. albicans. This ROS-stimulating effect of the supernatant ofS. schenkii was heat stable but not dialyzable. These findings suggest the possible role of ROS produced by infiltrated PMNs in the inflammatory skin lesions induced byS. schenkii.
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References
Allen RC & Loose LD. Phagocytic activation of a luminol-dependent chemilumenescence in rat alveolar and peritoneal macrophages. Biochem Biophys Res Commun 1976; 69:245–252.
Babior BM, Kipnes RS, Curnette JT. Biological defence mechanisms. The production by leukocytes of superoxide, a potential bactericidal agent. J Clin Invest 1973; 52:741–744.
Below PW, Rossenberg EW, Jennings BR. Activation of the alternative pathways of complement byMalassezia ovalis (pityrosporum ovale). Mycopathologia 1980; 70:187–191.
Cohen PP. Suspending media for normal tissues. In: Umbreit WW, Burris RH, Stauffer JF eds. Monocytometric technique and tissue metabolism, 3rd ed. Minneapolis: Burgress Publishing 1957; 149–150.
Conant NF et al. Manual of Clinical Mycology. 3rd Ed, Philidelphia: WB Saunders Co., 1971.
Diamond RD, May JE, Kane M, Frank M, Bennet JE. The role of the classical and alternate complement pathway in host defenses against cryptococcus neoformans infection. J Immunol 1974; 112:2260–2270.
Hachisuka H, Sasai Y. Development of experimental sporotrichosis in normal and modified animals. Mycopathologia 1982; 76:79–82.
Hachisula H, Tajiri S, Hongo K, Sasai Y. Polymorphonuclear leukocytes chemotaxis in sporortrichosis. Mythopathologia 1982; 79:39–41.
Johnston RB Jr, Lehmeyer JE. Elaboration of toxic oxygen by-products by neutrophils in a model of immune complex disease. J Chin Invest 1976; 57:836:841.
Klebanoff SJ, Rosen H. Ethylene formation by polymorphonuclear leukocytes. J Exp Med 1978; 148:490–506.
Lever WF, Schaumberg-Lever G. Histopathology of the skin. 6th Ed. Philadelphia: JB Lippincott 1983; 346–347.
Mackinnon JE, Conti-Diaz IA. The effect of temperature on sporotrichosis. Sabouraudia 1962; 2;56–59.
Myachi Y, Yanasie K, Imamura S, Niwa Y. Increased hydroxyl radical generation by normal polymorphonuclear leukocytes incubated in sera from patients with leukocytoclastic vasculitis. Arch Dermatol Res 1982; 274:65–71.
Miyachi M, Niwa Y. Effects of psoriatic sera on the generation of oxygen intermediates by normal polymorphonuclear leukocytes. Arch Dermatol Res 1983; 275:23–26.
Miyachi Y, Imamura S, Niwa Y. Erythema multiforme: A possible role of increased reactive oxygen species. J Clin Lab Immunol 1986; 19:11–14.
Müller-Peddinghaus R. In vitro determination of phagocyte activity by luminol- and lucigenin-amplified chemiluminescence. Int J Immunopharmacol 1984; 6:455–466.
Niwa Y, Miyake S, Sakane T, Shingu M, Yokoyama M. Autooxidative damage in Behçet's disease — endothelial cell damage following the elevated oxygen radicals generated by stimulated neutrophils. Clin Exp Immunol 1982; 49:247–255.
Niwa Y, Sohmiya K. Enhanced neutrophilic functions in mucocutaneous lymph node syndrome with special reference to the possible role of increased oxygen intermediates generation in auto-oxidative pathogenesis of coronary thromboarteritis. J Pediatr 1984; 104:56–60.
Niwa Y, Sohmiya K, Miyachi Y, Kanoh T, Sakane T. Luminol-independent chemiluminescence by phagocytes is markedly enhanced by dexamethascone, but not by other glucorticoids. Inflammation, in press.
Ray T, Wuepper KD. Activation of the alternative (properdin) pathway of complement byCandida albicans and related species. J Invest Dermatol 1976; 67:700–703.
Skosey JL, Chow DC, Damgaard E, Sorensen LB. Effects of Cytochalasin B on the response of human polymorphonuclear leukocytes to zymosan. J Cell Biol 1973; 57:237–240.
Swan JW, Dahl MV, Coppo PA, Hammerschmidt DE. Complement activation by Trichophyton rubrum. J Invest Dermatol 1983; 80:156–168.
Torinuki W, Okohchi K, Takematsu H, Tagami H. Activation of the alternative complement pathway by Fonsecaea pedrosoi. J Invest Dermatol 1984; 83:308–310.
Torinku W, Tagami H. Complement activation bySporothrix schenkii. Arch Dermatol Res 1985; 277:332–333.
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Yoshioka, A., Miyachi, Y., Imamura, S. et al. The effect of the supernatants obtained fromSporothrix schenkii andCandida albicans culture on the generation of reactive oxygen species by polymorphonuclear leukocytes. Mycopathologia 100, 43–48 (1987). https://doi.org/10.1007/BF00769567
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DOI: https://doi.org/10.1007/BF00769567