Abstract
Three classes of inner mitochondrial membrane (IMM) channel activities have been defined by direct measurement of conductance levels in membranes with patch clamp techniques in 150 mM K Cl. The “107 pS activity” is slightly anion selective and voltage dependent (open with matrix positive potentials). “Multiple conductance channel” (MCC) activity includes several levels from about 40 to over 1000 pS and can be activated by voltage or Ca2+. MCC may be responsible for the Ca2+-induced permeability transition observed with mitochondrial suspensions. A “low conductance channel” (LCC) is activated by alkaline pH and inhibited by Mg2+. LCC has a unit conductance of about 15 pS and may correspond to the inner membrane anion channel, IMAC, which was proposed from results obtained from suspension studies. All of the IMM channels defined thus far appear to be highly regulated and have a low open probability under physiological conditions. A summary of what is known about IMM channel regulation and pharmacology is presented and possible physiological roles of these channels are discussed.
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Amchenkova, A. A., Bakeeva, L. E., Chentsov, Y. S., Skulachev, V. P., and Zorov, D. B. (1988).J. Cell Biol. 107, 481–495.
Antonenko, Yu N., Kinnally, K. W., and Tedeschi, H. (1991a).J. Membr. Biol. 124, 151–158.
Antonenko, Yu N., Kinnally, K. W., Perini, S., and Tedeschi, H. (1991b).FEBS Lett. 285, 89–93.
Bakeeva, L. E., Chenstov, Yu. S., and Skulachev, V. P. (1983).J. Mol. Cell. Cardiol. 15, 413–420.
Beavis, A. D., and Garlid, K. D. (1987).J. Biol. Chem. 262, 15085–15093.
Beavis, A. D. (1989).J. Biol. Chem. 264, 1508–1515.
Beavis, A. D., and Powers, M. F. (1989).J. Biol. Chem. 264, 17148–17155.
Beavis, A. D. (1992).J. Bioenerg. Biomembr. 24, 77–90.
Bertl, A., and Slayman, C. (1991).Biophys. J. 59, 391a.
Benz, R., and Brdiczka, D. (1992).J. Bioenerg. Biomembr. 24, 33–40.
Blatz, A. L., and Magleby, K. L. (1983).Biophys. J. 43, 237–241.
Campo, M. L., Kinnally, K. W., and Tedeschi, H. (1990). 634th Meeting of the Biochemical Society Abstracts.
Costa, G., Kinnally, K. W., and Diwan, J. J. (1991).Biochem. Biophys. Res. Commun. 175, 305–310.
Decker, G. L., and Greenawalt, J. W. (1977).J. Ultrastruct. Res. 59, 44–56.
Fagian, M. M., Pereira-da-Silva, L., Martins, I. S., and Vercesi, A. E. (1990).J. Biol. Chem. 265, 19955–19960.
Garlid, K. D., and Beavis, A. D. (1986).Biochim. Biophys. Acta 853, 187–204.
Gray, P. T. A., Bevan, S., and Ritchie, J. M. (1984).Proc. R. Soc. London B 221, 395–409.
Gunter, T. E., and Pfeiffer, D. R. (1990).Am. J. Physiol. C, Cell Physiol. 27, 755–786.
Henry, J.-P., Chich, J.-F., Goldschmidt, D., and Thieffry, M. (1989).J. Membr. Biol. 112, 139–147.
Holden, M. J., and Colombini, M. (1988).FEBS Lett. 241, 105–109.
Hunter, D. R., Haworth, R. A., and Southward, J. H. (1976).J. Biol. Chem. 251, 5069–5077.
Inoue, I., Nagase, H., Kishi, K., and Higuti, T. (1991).Nature (London)352, 244–247.
Jeng, A. Y., and Shamoo, A. E. (1980),J. Biol. Chem. 255, 6897–6903.
Jezek, P., and Garlid, K. D. (1990)J. Biol. Chem. 265, 19303–19311.
Kinnally, K. W., Campo, M. L., and Tedeschi, H. (1989).J. Bioenerg. Biomembr. 21, 497–506.
Kinnally, K. W., Zorov, D. B., Antonenko, Yu N., and Perini, S. (1991).Biochem. Biophys. Res. Commun. 176, 1183–1188.
Klitsch, T., and Siemen, D. (1991).J. Membr. Biol. 122, 69–75.
Kolhardt, M., and Fichtner, H. (1988).J. Membr. Biol. 102, 105–119.
Krouse, M. E., Schneider, G. T., and Gage, P. W. (1986).Nature (London)319, 58–60.
Last, T. A., Gantzer, M. L., and Tyler, C. D. (1983).J. Biol. Chem. 258, 2399–2404.
Meves, H., and Nagy, K. (1989).Biochim. Biophys. Acta 988, 99–105.
Mirazabekov, T., and Ermishkin, L. N. (1989).FEBS Lett. 249, 375–78.
Mironova, G. D., Fedotcheva, N. I., Makarov, P. R., Pronevich, L. A., and Mironov, G. P. (1981).Biofizika 26, 451–457.
Mironova, G. D., Sirota, T. J., Pronevich, L. A., Trofimenko, N. V., Mironov, G. P., Grigorjev, P. A., and Kandrashova, M. N. (1982),J. Bioenerg. Biomembr. 14, 213–225.
Moczydlowski, E. (1986). InIon Channel Reconstitution (Miller, C. ed.), Plenum Press, New York, pp. 75–113.
Moran, O., Sandri, G., Panfili, E., Stühmer, W., and Sorgato, M. C. (1990).J. Biol. Chem. 265, 908–913.
Moran, O., and Sorgato, M. C. (1992).J. Bioenerg. Biomembr. 24, 91–98.
Panfili, E., Sandri, G., Liut, G., Stancher, B., and Sottocasa, G. L. (1983). InCa 2+ Binding Proteins (de Bernard, B., Sottocasa, G. L., Sandri, G., Carafoli, E., Taylor, A. N., Vanaman, T. C., and Williams, R. J. P., eds.), Elsevier, Amsterdam, New York, pp. 347–364.
Petronilli, V., Szabò, I., and Zoratti, M. (1989).FEBS Lett. 259, 137–143.
Powers, M. F., and Beavis, A. D. (1991)Biophys. J. 59, 596a.
Sachs, F. (1983). InSingle-Channel Recording (Sakmann, B., and Neher, E., eds.), Plenum Press, New York, 365–376.
Sandri, G., Siagri, M., and Panfili, E. (1988).Cell Calcium 9, 159–165.
Schwaiger, M., Herzog, V., and Neupert, W. (1987).J. Cell Biol. 105, 235–246.
Selwyn, M. J., Dawson, A. P., and Fulton, D. V. (1979).Biochem. Soc. Trans. 7, 216–219.
Singer, S. J., Maher, P. A., and Yaffe, M. P. (1978).Proc. Natl. Acad. Sci. USA 84, 1015–1019.
Sorgato, M. C., Keller, B. U., and Stühmer, W. (1987).Nature (London)330, 498–500.
Sorgato, M. C., Moran, O., De Pinto, V., Keller, B. U., and Stühmer, W. (1989).J. Bioenerg. Biomembr. 21, 485–496.
Szabó, I., and Zoratti, M. (1991).J. Biol. Chem. 266, 3376–3379.
Szabó, I., and Zoratti, M. (1992).J. Bioenerg. Biomembr. 24, 111–118.
Tedeschi, H. (1981).Biochim. Biophys. Acta 639, 157–196.
Tedeschi, H., Mannella, C., and Bowman, C. (1987).J. Membr. Biol. 97, 21–29.
Zorov, D. B., Kinnally, K. W., Perini, S., and Tedeschi, H. (1991).Biophys. J. 59, 216a.
Zorov, D. B., Kinnally, K. W., and Tedeschi, H. (1992).J. Bioenerg. Biomembr. 24, 119–124.
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Kinnally, K.W., Antonenko, Y.N. & Zorov, D.B. Modulation of inner mitochondrial membrane channel activity. J Bioenerg Biomembr 24, 99–110 (1992). https://doi.org/10.1007/BF00769536
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DOI: https://doi.org/10.1007/BF00769536