Abstract
The mitochondrial inner membrane possesses an anion channel (IMAC) which mediates the electrophoretic transport of a wide variety of anions and is believed to be an important component of the volume homeostatic mechanism. IMAC is regulated by matrix Mg2+ (IC50=38 µM at pH 7.4) and by matrix H+ (pIC50=7.7). Moreover, inhibition by Mg2+ is pH-dependent. IMAC is also reversibly inhibited by many cationic amphiphilic drugs, including propranolol, and irreversibly inhibited byN,N′-dicyclohexylcarbodiimide. Mercurials have two effects on its activity: (1) they increase the IC50 values for Mg2+, H+, and propranolol, and (2) they inhibit transport. The most potent inhibitor of IMAC is tributyltin, which blocks anion uniport in liver mitochondria at about 1 nmol/mg. The inhibitory dose is increased by mercurials; however, this effect appears to be unrelated to the other mercurial effects. IMAC also appears to be present in plant mitochondria; however, it is insensitive to inhibition by Mg2+, mercurials, andN,N′-dicyclohexylcarbodiimide. Some inhibitors of the adenine nucleotide translocase also inhibit IMAC, including Cibacron Blue, agaric acid, and palmitoyl CoA; however, atractyloside has no effect.
Similar content being viewed by others
References
Aldridge, W. N., and Street, B. W. (1970).Biochem. J. 118 171–179.
Aquila, H., Link, T. A., and Klingenberg, M. (1987).FEBS Lett. 212 1–9.
Azzi, A., and Azzone, G. F. (1966).Biochim. Biophys. Acta 120 466–468.
Azzi, A., and Azzone, G. F. (1967).Biochim. Biophys. Acta 131 468–478.
Azzi, A., Casey, R. P., and Nalecz, M. J. (1984).Biochim. Biophys. Acta 768 209–226.
Beavis, A. D. (1989a).J. Biol. Chem. 264 1508–1515.
Beavis, A. D. (1989b).Eur. J. Biochem. 185 511–519.
Beavis, A. D. (1991).Biochim. Biophys. Acta 1063 111–119.
Beavis, A. D., and Garlid, K. D. (1983).Fed. Proc. 42 1945.
Beavis, A. D., and Garlid, K. D. (1987).J. Biol. Chem. 262 15085–15093.
Beavis, A. D., and Garlid, K. D. (1988).J. Biol. Chem. 263 7574–7580.
Beavis, A. D., and Garlid, K. D. (1990).J. Biol. Chem. 265 2538–2545.
Beavis, A. D., and Powers, M. F. (1989).J. Biol. Chem. 264 17148–17155.
Beavis, A. D., and Vercesi, A. E. (1992).J. Biol. Chem. 267, in press.
Beavis, A. D., Brannan, R. D., and Garlid, K. D. (1985).J. Biol. Chem. 260 13424–13433.
Bogucka, K., and Wojtczak, L. (1979).FEBS Lett. 100 301–304.
Brierley, G. P. (1970).Biochemistry 9 697–707.
Brierley, G. P., and Jung, D. W. (1988).J. Bioenerg. Biomembr. 20 193–209.
Brierley, G. P., Jurkowitz, M. S., Chavez, E., and Jung, D. W. (1977).J. Biol. Chem. 252 7932–7939.
Day, D. A., and Wiskich, J. T. (1984).Physiol. Veg. 22 241–261.
Diwan, J. J. (1982).J. Bioenerg. Biomembr. 14 15–22.
Diwan, J. J. (1988).Biochim. Biophys. Acta 895 155–165.
Diwan, J. J., DeLucia, A., and Rose, P. E. (1983).J. Bioenerg. Biomembr. 15 277–288.
Douce, R., and Neuberger, M. (1989).Annu. Rev. Plant Physiol. 40 371–414.
Fonyo, A. (1979).Pharmacol. Ther. 1 627–645.
Garlid, K. D. (1988). InIntegration of Mitochondrial Function (Lemasters, J. J., Hackenbrock, C. R., Thurman, R. G., and Westerhoff, H. V., eds.), Plenum Press, New York, pp. 259–278.
Garlid, K. D., and Beavis, A. D. (1985).J. Biol. Chem. 260 13434–13441.
Garlid, K. D., and Beavis, A. D. (1986).Biochim. Biophys. Acta 853 187–204.
Gauthier, L. M., and Diwan, J. J. (1979).Biochem. Biophys. Res. Commun. 87 1072–1079.
Gunter, T. E., and Pfeiffer, D. R. (1990).Am. J. Physiol. 258 C755–C786.
Halle-Smith, S. C., Murray, A. G., and Selwyn, M. J. (1988).FEBS Lett. 236 155–158.
Hanson, J. B., and Hodges, T. K. (1967). INCurrent Topics in Bioenergetics (Sanadi, D. R., ed.). Vol. 2, Academic Press, New York, pp. 65–98.
Hanson, J. B., and Koeppe, D. E. (1975). InIon Transport in Plant Cells and Tissues (Baker, D. A., and Hall, J. L., eds.), North-Holland, Amsterdam, pp. 79–99.
Heber, U., and Heldt, H. W. (1981).Annu. Rev. Plant Physiol. 32 139–168.
Hegazy, M. G., Mahdi, F., Li, X., Gui, G., Mironova, G., Beavis, A., and Garlid, K. D. (1991).Biophys. J. 59, 136a.
Hensley, J. R., and Hanson, J. B. (1975).Plant Physiol. 56 13–18.
Huber, S. C., and Moreland, D. E. (1979).Plant Physiol. 64 115–119.
Jezek, P., Beavis, A. D., DiResta, D. J., Cousino, R. N., and Garlid, K. D. (1989).Am. J. Physiol. 257 C1142–C1148.
Jung, D. W., and Brierley, G. P. (1979).Plant Physiol. 64 948–953.
Jung, D. W., Shi, G.-Y., and Brierley, G. P. (1980).J. Biol. Chem. 255 408–412.
Kromer, S., and Heldt, H. W. (1991).Biochim. Biophys. Acta 1057 42–50.
LaNoue, K. F., and Schoolwerth, A. C. (1979).Annu. Rev. Biochem. 48 871–922.
Martin, W. H., Beavis, A. D., and Garlid, K. D. (1984).J. Biol. Chem. 258 2062–2065.
Murray, A. G., Halle-Smith, S. C., and Selwyn, M. J. (1988).E.B.E.C. Rep. 5 206.
Neslund, G. G., Miara, J. E., Kang, J. J., and Dahms, A. S. (1984). InCurrent Topics in Cellular Regulation, Vol. 24, Academic Press, New York, pp. 447–469.
Nobel, R. S. (1975). InIon Transport in Plant Cells and Tissues (Baker, D. A., and Hall, J. L., eds), North-Holland, Amsterdam, pp. 101–124.
Powers, M. F., and Beavis, A. D. (1990).Biophys. J. 57, 179a.
Powers, M. F., and Beavis, A. D. (1991).J. Biol. Chem. 266 17250–17256.
Reynafarje, B., and Lehninger, A. L. (1978).Proc. Natl. Acad. Sci. USA 75 4788–4792.
Runswick, M. J., Walker, J. E., Bisaccia, F., Iacobazzi, V., and Palmieri, F. (1990).Biochemistry 29 11033–11040.
Scott, K. M., Knight, V. A., Settlemire, C. T., and Brierley, G. P. (1970).Biochemistry 9 714–723.
Selwyn, M. J., (1972).Biochem. J. 130 65p–67p.
Selwyn, M. J. (1976). InOrganotin Compounds: New Chemistry and Applications (Zuckerman, J. J., ed.), Amer. Chem. Soc., Washington, D.C., pp. 204–226.
Selwyn, M. J., Dawson, A. P., Stockdale, M., and Gains, N. (1970).Eur. J. Biochem. 14 120–126.
Selwyn, M. J., Fulton, D. V., and Dawson, A. P. (1978).FEBS Lett. 96 148–151.
Selwyn, M. J., Dawson, A. P., and Fulton, D. V. (1979).Biochem. Soc. Trans. 7 216–219.
Selwyn, M. J., Ng, C. L. T., and Choo, H. L. (1990).FEBS Lett. 269 205–208.
Stockdale, M., Dawson, A. P., and Selwyn, M. J. (1970).Eur. J. Biochem. 15 342–351.
Stoner, C. D., and Hanson, J. B. (1966).Plant Physiol. 41 255–266.
Warhurst, I. W., Dawson, A. P., and Selwyn, M. J. (1982).FEBS Lett. 149 249–252.
Watling, A. S., and Selwyn, M. J. (1970).FEBS Lett. 10 139–142.
Yoshida, K. (1968).J. Fac. Sci. Univ. Tokyo III 10 63–82.
Zernig, G., Graziadei, I., Moshammer, T., Zech, C., Reider, N., and Glossman, H. (1990).Mol. Pharmacol. 38 362–369.
Zoglowek, C., Kromer, S., and Heldt, H. W. (1988).Plant Physiol. 87 109–115.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Beavis, A.D. Properties of the inner membrane anion channel in intact mitochondria. J Bioenerg Biomembr 24, 77–90 (1992). https://doi.org/10.1007/BF00769534
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00769534