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Cellular and Molecular Neurobiology

, Volume 11, Issue 2, pp 289–294 | Cite as

Developmental changes of neuron-specific enolase mRNA in primary cultures of rat neurons

  • Italia Di Liegro
  • Alessandro Cestelli
  • Giovanna Barbieri
  • Agata Giallongo
Short Communication

Summary

  1. 1.

    The level of mRNAs for neuron-specific enolase (NSE) and nonneuronal enolase (NNE) was studied in developing rat brain and in pure neuronal cultures of corresponding ages treated or not treated with triiodothyronine (T3).

     
  2. 2.

    In brain cortices both messages are already detectable at the earliest age (embryonal day 16; E16). During development the mRNA for NNE remains at a steady level, with a transient decline at postnatal day 5 (P5).

     
  3. 3.

    On the other hand, NSE mRNA follows a biphasic curve: the signal increases threefold from E-16 to P0 and threefold from P5 to P18, with a plateau between P0 and P5.

     
  4. 4.

    In neuronal cultures the NNE message is present at a constant level until day 10 and declines sharply thereafter, while in T3-treated cultures it reaches a minimum beforehand.

     
  5. 5.

    The NSE mRNA, on the other hand, increases continuously throughout the whole culture life span, and a slightly higher level is observed in T3-treated cells during the first ten days.

     

Key words

neuronal cultures neuron-specific enolase nonneuronal enolase thyroid hormones 

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Copyright information

© Plenum Publishing Corporation 1991

Authors and Affiliations

  • Italia Di Liegro
    • 1
  • Alessandro Cestelli
    • 1
  • Giovanna Barbieri
    • 1
  • Agata Giallongo
    • 2
  1. 1.Dipartimento di Biologia Cellulare e dello Sviluppo “Alberto Monroy,”Università degli StudiPalermoItaly
  2. 2.Istituto di Biologia dello SviluppoConsiglio Nazionale delle RicerchePalermoItaly

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