Cellular and Molecular Neurobiology

, Volume 11, Issue 2, pp 289–294 | Cite as

Developmental changes of neuron-specific enolase mRNA in primary cultures of rat neurons

  • Italia Di Liegro
  • Alessandro Cestelli
  • Giovanna Barbieri
  • Agata Giallongo
Short Communication


  1. 1.

    The level of mRNAs for neuron-specific enolase (NSE) and nonneuronal enolase (NNE) was studied in developing rat brain and in pure neuronal cultures of corresponding ages treated or not treated with triiodothyronine (T3).

  2. 2.

    In brain cortices both messages are already detectable at the earliest age (embryonal day 16; E16). During development the mRNA for NNE remains at a steady level, with a transient decline at postnatal day 5 (P5).

  3. 3.

    On the other hand, NSE mRNA follows a biphasic curve: the signal increases threefold from E-16 to P0 and threefold from P5 to P18, with a plateau between P0 and P5.

  4. 4.

    In neuronal cultures the NNE message is present at a constant level until day 10 and declines sharply thereafter, while in T3-treated cultures it reaches a minimum beforehand.

  5. 5.

    The NSE mRNA, on the other hand, increases continuously throughout the whole culture life span, and a slightly higher level is observed in T3-treated cells during the first ten days.


Key words

neuronal cultures neuron-specific enolase nonneuronal enolase thyroid hormones 


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  1. Cestelli, A., Savettieri, G., Ferraro, D., and Vitale, F. (1985). Formulation of a novel synthetic medium for selectively culturing rat CNS neurons.Dev. Brain Brs. 22219–227.Google Scholar
  2. Cestelli, A., Di Liegro, I., Castiglia, D., Gristina, R., Ferraro, D., Salemi, G., and Savettieri, G. (1987). Triiodothyronine-induced shortening of chromatin repeat length in neurons cultured in a chemically defined medium.J. Neurochem. 481053–1059.Google Scholar
  3. Chomczynski, P., and Sacchi, N. (1987). Single-step method of RNA isolation by acid guanidinium-thiocyanate-phenol-chloroform extraction.Anal. Biochem. 162156–159.Google Scholar
  4. Di Liegro, I., Savettieri, G., and Cestelli, A., (1987). Cellular mechanism of action of thyroid hormones.Differentiation 35165–175.Google Scholar
  5. Dussault, J. H., and Ruel, J. (1987). Thyroid hormones and brain development.Ann. Rev. Physiol. 49321–334.Google Scholar
  6. Erickson, J. M., Rushford, C. L., Dorney, D. J., and Wilson, G. N. (1981). Structure and variation of human ribosomal DNA: Molecular analysis of cloned fragments.Gene 161–9.Google Scholar
  7. Feinberg, A. P., and Volgestein, B. (1983). A technique for radiolabeling DNA restriction endonuclease fragments to high specificity.Anal Biochem. 1326–13.Google Scholar
  8. Forss-Petter, S., Danielson, P., and Sutcliffe, J. G. (1986). Neuron-specific enolase: Complete structure of rat mRNA, multiple transcriptional start sites, and evidence suggesting posttranscriptional control.J. Neurosci. Res. 16141–156.Google Scholar
  9. Giallongo, A., Yon, J., and Fried, M. (1989). Ribosomal protein L7a is encoded by a gene (Surf-3) within the tightly clusered mouse surfeit locus.Mol. Cell. Biol. 9224–231.Google Scholar
  10. Jacobson, M. (1978).Developmental Neurobiology, Plenum Press, New York.Google Scholar
  11. Lucas, M., Lamandé, N., Lazar, M., Gros, F., and Legault-Demare, L. (1988). Developmental expression of alpha- and gamma-enolase subunits and mRNA sequences in the mouse brain.Dev. Neurosci. 1091–98.Google Scholar
  12. Marangos, P. J., and Schmechel, D. E. (1987). Neuron specific enolase, a clinically useful marker for neurons and neuroendocrine cells.Ann. Rev. Neurosci. 10269–295.Google Scholar
  13. Marangos, P. J., Schemechel, D. E., Parma, A. M., and Goodwin, F. K. (1980). Developmental profile of neuron specific (NSE) and non-neuronal (NNE) enolase.Brain Res. 190185–193.Google Scholar
  14. Oliva, D., Barba, G., Barbieri, G., Giallongo, A., and Feo, S. (1989). Cloning, expression and sequence homologies of cDNA for human gamma enolase.Gene 79355–360.Google Scholar
  15. Savettieri, G., Di Liegro, I., and Cestelli, A. (1989). Action of thyroid hormones on developing CNS at the molecular level. InBiological Aspects of Neuron Activity. (V. Bonavita and F. Piccoli, Eds.), Fidia Biomedical Information, Padova, pp. 17–27.Google Scholar

Copyright information

© Plenum Publishing Corporation 1991

Authors and Affiliations

  • Italia Di Liegro
    • 1
  • Alessandro Cestelli
    • 1
  • Giovanna Barbieri
    • 1
  • Agata Giallongo
    • 2
  1. 1.Dipartimento di Biologia Cellulare e dello Sviluppo “Alberto Monroy,”Università degli StudiPalermoItaly
  2. 2.Istituto di Biologia dello SviluppoConsiglio Nazionale delle RicerchePalermoItaly

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