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An alignment of 17 deduced protein sequences from plant, fungi, and ciliate H+-ATPase genes

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Abstract

Seventeen protein sequences of H+-ATPases from plants (Arabidopsis thaliana, Nicotiana plumbaginifolia, Lycopersicum esculentum), fungi (Sacharomyces cerevisiae, Schizosaccharomyces pombe, Zygosaccharomyces rouxii, Neuropora crassa, Candida albicans), and a parasitic ciliate (Leishmania donovani) have been aligned. Twenty sequence fragments were identified which were conserved in H+-, Na+/K+-, and Ca++ plasma membrane-ATPases. In addition, a total of 118 residues not located in these fragments were found to be conserved in all H+-ATPases. Among those, 38 amino acid residues were screened out as being priority targets for site-directed mutagenesis experiments aimed at the identification of the amino acid residues specifically involved in cation specificity.

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References

  • Addison, R. (1986).J. Biol. Chem. 261, 14896–14901.

    Google Scholar 

  • Addison, R., and Scarborough, G. A. (1981).J. Biol. Chem. 256, 13165–13171.

    Google Scholar 

  • Boutry, M., Michelet, B., and Goffeau, A. (1989).Biochim. Biophys. Res. Commun. 162, 567–574.

    Google Scholar 

  • Bowman, B. J., and Bowman, E. J. (1986).J. Membr. Biol. 94, 83–93.

    Google Scholar 

  • Bowman, B. J., Blasco, F., and Slayman, C. W. (1981).J. Biol. Chem. 256, 12343–12349.

    Google Scholar 

  • Clarke, D. M., Loo, T. W., Inesi, G., and MacLennan, D. H. (1989).Nature (London)339, 476–478.

    Google Scholar 

  • Davis, B. D., and Hammes, G. G. (1989).J. Biol. Chem. 264, 370–374.

    Google Scholar 

  • Deisenhofer, J., Epp, O., Miki, K., Huber, R., and Michel, H. (1985).Nature (London)318, 618–624.

    Google Scholar 

  • Dufour, J. P., and Goffeau, A. (1978).J. Biol. Chem. 253, 7026–7032.

    Google Scholar 

  • Ewing, N. N., Wimmers, L. E., Meyer, D. J., Chetelat, R. T., and Bennett, A. B. (1990).Plant Physiol. 94, 1874–1881.

    Google Scholar 

  • Fasman, G. D., and Gilbert, W. A. (1990).Trends Biochem. Sci. 15, 89–92.

    Google Scholar 

  • Feng, D. F., Johnson, M. S., and Doolittle, R. F. (1985).J. Mol. Evol. 21, 112–125.

    Google Scholar 

  • Foury, F., Amory, A., and Goffeau, A. (1981).Eur. J. Biochem. 119, 395–400.

    Google Scholar 

  • Gaber, R. (1992).International Reviews of Cytology: Membrane Transporters and Receptors, in press.

  • Ghislain, M., and Goffeau, A. (1991).J. Biol. Chem. 266, 18276–18279.

    Google Scholar 

  • Ghislain, M., Schlesser, A., and Goffeau, A. (1987).J. Biol. Chem. 262, 17549–17555.

    Google Scholar 

  • Goffeau, A. (1987). InMolecular Basis of Membrane Transport, UNESCO Workshop (Kotyk, A., ed.), printed for UNESCO by Czechoslovak Academy of Sciences, Prague pp. 32–37.

  • Goffeau, A. (1988).Brazilian J. Med. Biol. Res. 21, 1233–1240.

    Google Scholar 

  • Goffeau, A., and Boutry, M. (1986).Microbiol. Sci. 3, 164–167.

    Google Scholar 

  • Goffeau, A., and Green, N. M. (1990). InMonovalent Cations in Biological Systems (Pasternak, C. A. ed.), CRC Press, Boca Raton, Florida, pp. 155–169.

    Google Scholar 

  • Goffeau, A., and Slayman, C. W. (1981).Biochim. Biophys. Acta 639, 197–223.

    Google Scholar 

  • Goffeau, A., Coddington, A., and Schlesser, A. (1989). InMolecular Biology of the Fission Yeast (Nasim, A., Young, P., and Johnson, B., eds.), Adademic Press, Orlando, Florida, pp. 397–429.

    Google Scholar 

  • Green, N. M. (1989).Biochem. Soc. Trans. 17, 819–822.

    Google Scholar 

  • Hager, K. M., Mandala, S. M., Davenport, J. W., Speicher, D. W., Benz, E. J., and Slayman, C. W. (1986).Proc. Natl. Acad. Sci. USA 83, 7693–9697.

    Google Scholar 

  • Harper, J. F., Surowy, T. K., and Sussmann, M. R. (1989).Proc. Natl. Acad. Sci. USA 86, 1234–1238.

    Google Scholar 

  • Harper, J. F., Manney, L., DeWitt, N. D., Yoo, M. H., and Sussmann, M. R. (1990).J. Biol. Chem. 265, 13601–13608.

    Google Scholar 

  • Hennessey, J. P., Jr., and Scarborough, G. A. (1990).J. Biol. Chem. 265, 532–537.

    Google Scholar 

  • Higgins, D. G., and Sharp, P. M. (1988).Gene 73, 237–244.

    Google Scholar 

  • Houlné, G., and Boutry, M., personal cummunication.

  • Klein, P., Kanehisa, M., and DeLisi, C. (1985).Biochim. Biophys. Acta 815, 468–476.

    Google Scholar 

  • Koland, J. G., and Hammes, G. G. (1986).J. Biol. Chem. 261, 5936–5942.

    Google Scholar 

  • Malpartida, F., and Serrano, R. (1980).FEBS Lett. 111, 69–72.

    Google Scholar 

  • Mandala, S. M., and Slayman, C. W. (1989).J. Biol. Chem. 264, 16276–16281.

    Google Scholar 

  • Meade, J. C., Shaw, J., Lemaster, S., Gallagher, G., and Stringer, J. R. (1987).Mol. Cell. Biol. 7, 3937–3946.

    Google Scholar 

  • Monk, B. C., Kurtz, M. B., Marrinan, J. A., and Perlin, D. (1991).J. Bacteriol. 173, 6826–6836.

    Google Scholar 

  • Morieau, L., and Boutry, M., personal communication.

  • Nakamoto, R. K., and Slayman, C. W. (1989).J. Bioenerg. Biomembr. 21, 621–632.

    Google Scholar 

  • Nakamoto, R. K., Rao, R., and Slayman, C. W. (1991).J. Biol. Chem. 266, 7940–7949.

    Google Scholar 

  • Pardo, J. M., and Serrano, R. (1989).J. Biol. Chem. 264, 8557–8562.

    Google Scholar 

  • Perez, C., Michelet, B., Ferrant, V., Bogaerts, P., and Boutry, M. (1992).J. Biol. Chem.,267, 1204–1211.

    Google Scholar 

  • Perlin, D. S., Harris, S. L., Seto-Young, D., and Haber, J. E. (1989).J. Biol. Chem. 264, 21857–21864.

    Google Scholar 

  • Portillo, F., and Serrano, R. (1988).EMBO J. 7, 1793–1798.

    Google Scholar 

  • Scarborough, G. A. (1985).Microbiol. Rev. 49, 214–231.

    Google Scholar 

  • Scarborough, G. A. (1992), InMolecular Aspects of Membrane Proteins (de Pont, J. J. H. H. M., ed.), Elsevier Science Publishers, Amsterdam, New York, in press.

    Google Scholar 

  • Schlesser, A., Ulaszewski, S., Ghislain, M., and Goffeau, A. (1988).J. Biol. Chem. 263, 19480–19487.

    Google Scholar 

  • Serrano, R. (1984).Curr. Top. Cell. Regul. 23, 87–126.

    Google Scholar 

  • Serrano, R. (1988).Biochim. Biophys. Acta 947, 1–28.

    Google Scholar 

  • Serrano, R. (1989).Annu. Rev. Plant Physiol. Plant Mol. Biol. 40, 61–94.

    Google Scholar 

  • Serrano, R. (1991). InThe Molecular and Cellular Biology of the Yeast Saccharomyces, Genome Dynamics, Protein Synthesis, and Energetics, (Broach, J. R., Pringle, J. R., and Jones, E., eds.), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, Chap. 9, pp. 523–585.

    Google Scholar 

  • Serrano, R., Kielland-Brandt, M. C., and Fink, G. R. (1986).Nature (London)319, 689–693.

    Google Scholar 

  • Shull, G. E., and Greeb, J. (1988).J. Biol. Chem. 263, 8646–8657.

    Google Scholar 

  • Shull, G. E., Schwartz, A., and Lingrel, J. B. (1985).Nature (London)316, 691–695.

    Google Scholar 

  • Slayman, C. L. (1987).J. Bioenerg. Biomembr. 19, 1–20.

    Google Scholar 

  • Subrahmanyeswara Rao, U., Hennessey, J. P., and Scarborough, G. A. (1991).J. Biol. Chem. 266, 14740–14746.

    Google Scholar 

  • Van Dyck, L., Petretski, J. H., Wolasker, H., Rodrigues, G., Schlesser, A., Ghislain, M., and Goffeau, A. (1990).Eur. J. Biochem. 194, 785–790.

    Google Scholar 

  • Wach, A., Ahlers, J., and Gräber, P. (1990).Eur. J. Biochem. 189, 675–682.

    Google Scholar 

  • Watanabe, Y., Shiramizu, M., and Tamai, Y. (1991).J. Biochem. 110, 237–240.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Unité de Biochimie Physiologique, Université Catholique de Louvain, Place Croix du Sud 2–20, B-1348, Louvain-la-Neuve, Belgium

    Achim Wach, Alain Schlesser & André Goffeau

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  1. Achim Wach
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  2. Alain Schlesser
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  3. André Goffeau
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Wach, A., Schlesser, A. & Goffeau, A. An alignment of 17 deduced protein sequences from plant, fungi, and ciliate H+-ATPase genes. J Bioenerg Biomembr 24, 309–317 (1992). https://doi.org/10.1007/BF00768851

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  • DOI: https://doi.org/10.1007/BF00768851

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