Abstract
DR gene products are commonly thought to be involved in the induction of the mixed lymphocyte reaction (MLR). However, very little is known about the role of HLA DQ antigens in the MLR. To address this question, we introduced DQα andβ chain genes into mouse L cells, a human T -cell line, and a human premonocytoid cell line using a liposome-mediated transfer technique. The DQ α and DQ β genomic clones were isolated from a DR2 DQw1 and a DR3 DQw2 phage library, respectively. ThepSV2-Neo gene was introduced as a selection marker with both DQ α and DQ β . The resultant transfected cells were able to bind several HLA class II monoclonal antibodies. In addition, these cells were found to be efficient in stimulating peripheral blood lymphocyte proliferation under MLR-like conditions, implying a role for HLA-DQ molecules in HLA-D typing differences.
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References
Auffray, C., Lillei, J. W., Arnot, D., Grossberger, D., Kappes, D., and Strominger, J. L.: Isotypic and allotypic variation of the class II human histocompatibility antigenα chain genes.Nature 308: 327–333, 1984
Austin, P., Trowsdale, J., Rudd, C., Bodmer, W., Feldman, M., and Lamb, J.: Functional expression ofHLA-DP genes transfected into mouse fibroblasts.Nature 313: 61–64, 1985
Bach, F. H.: HLA class II genes/products—the HLA-D region.Immunol. Today 6: 89–94, 1985
Ben-Nun, A., Glimcher, L. H., Weis, J., and Seidman, J. G.: Functional expression of a cloned 1-Ak gene in B-lymphoma cells.Science 233: 825–828,1984
Bodmer, J. and Bodmer, W. F.: Histocompatibility 1984.Immunol. Today 5: 251–254, 1984
Boss, J. M. and Strominger, J. L.: Cloning and sequence analysis of the human major histocompatibility complex gene DC-3β.Proc. Natl. Acad. Sci. U.S.A. 81: 5199–5203, 1984
Brodsky, F. M.: A matrix approach to human class II histocompatibility antigens: Reactions of four monoclonal antibodies with the products of nine haplotypes.Immunogenetics 19: 179–194, 1984
Foley, G. E., Lazarus, H., Sidney, F., Uzman, B. G., Boone, B. A., and McCarthy, R. E.: Continuous culture of human lymphoblasts from peripheral blood of a child with acute leukemia.Cancer 18: 522–529, 1965
Folson, V., Gold, D. P., White, J., Marrack, P., Kappler, J., and Tonegawa, S.: Functional and inducible expression of a transfected murine class II major histocompatibility complex gene.Proc. Natl. Acad. Sci. U.S.A. 81: 2045–2049, 1984
Germain, R. N., Noreross, M. A., and Margulies, D. H.: Functional expression of a transfected murine class II MHC gene, Eβ.Nature 306: 190–194, 1983
Goodenow, R. S., McMillan, M., Nicolson, M., Sher, B. T., Eakle, K., Davidson, N., and Hood, L.: Identification of the class I genes of the mouse major histocompatibility complex by DNA-mediated gene transfer.Nature 300: 231–237, 1982
Goyert, S. M. and Silver, J.: Isolation of I-A subregion-like molecules from subhuman primates and man.Nature 294: 266–268, 1981
Gregerson, P. K., Shen, M., Song, Q., Merryman, P., Degar, S., Seki, T., Maccari, J., Goldberg, D., Murphy, H., Schwenzer, J., Wang, C. Y., Winchester, R. J., Nepom, G. T., and Silver, J.: Molecular diversity of HLA-DR4 haplotypes.Proc. Natl. Acad. Sci. U.S.A. 83: 2642–2646, 1986
Inoko, H., Ando, A., Kimura, M., and Tsuji, K.: Isolation and characteri-zation of the cDNA clones and genomic clones of the HLA class II antigens.In K. Tsuji (ed.):Proceeding of the 20th Annual Meeting of Japanese Society for Transplantation, pp. 188–220, Tokai University Press, Tokyo, 1985
Jaraquemada, D., Navarrete, C., Ollier, W., Awad, J., Okoye, R., and Festenstein, H.: HLA Dw specificity assignments are independent of HLA-DQ, HLA-DR, and other class II specificities and define a biologically important segregant series which strongly activates a functionally distinct T cell subset.Hum. Immunol. 16: 259–270, 1986
Kasahara, M., Takenouchi, T., Ikeda, H., Ogasawara, K., Okyuama, T., Ishikawa, N., Wakisaka, A., Kikuchi, Y., and Aizawa, M.: Serologic dissection of HLA-D specificities by the use of monoclonal antibodies. II. Distinction between HLA-Dw2 and HLA-Dw12.Immunogenetics 18: 525–536, 1983
Lawn, R. M., Fritsh, E. E., Parker, R. C., Blake, G., and Maniatis, T.: The isolation and characterization of linkedδ- andβ-globulin genes from a cloned library of human DNA.Cell 15: 1457–1174, 1978
Malissen, B., Peele-Price, M., Goverman, J. M., McMillar, M., White, J., Kapper, J., Marrack, P., Pierres, A., Pierres, M., and Hood, L.: Gene transfer of H-2 class II genes: Antigen presentation by mouse fibroblast and hamster B-cell lines.Cell 36: 319–327, 1984
Maniatis, T., Fritsch, E. F., and Sanbrook, J.: In situ hybridization of bacterial colonies of bacteriophage plaques.In T. Maniatis (ed.):Molecular Cloning, pp. 312–328, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1982
Nakatsuji, T., Moriya, K., Ando, A., Inoko, H., and Tsuji, K.: HLA-DQ structural polymorphism in HLA-DR2 associated HLA-D clusters.Hum. Immunol. 16: 157–162, 1986
O'Farrell, P. Z., Goodman, H. M., and O'Farrell, P. H.: High resolution two-dimensional electrophoresis of basic as well as acidic proteins.Cell 12: 1133–1142, 1977
Okada, K., Boss, J., Prentice, H., Spies, T., Mangler, R., Auffray,C., Lillie, J., Grossberger, D., and Strominger, J. L.: Gene organization of the DC and DX subregions of the human major histocompatibility complex.Proc. Natl. Acad. Sci. U.S.A. 82: 3410–3414, 1985
Pawelec, G. P., Shaw, S., Ziegler, A., Müller, C., and Werner, P.: Differential inhibition of HLA-D- or SB-directed secondary lymphoproliferative responses with monoclonal antibodies detecting human Ia-like determinant.J. Immunol. 129: 1070–1075, 1982
Rabourdin-Combe, C. and Mach, B.: Expression of HLA-DR antigens at the surface of mouse L cells co-transfected with cloned human genes.Nature 303: 670–674, 1983
Schaefer-Ridder, M., Wang, Y., and Hofschneider, P. H.: Liposomes as gene carriers: Efficient transformation of mouse L cells by thymidine kinase gene.Science 215: 166–168, 1982
Spielman, R., Lee, J. S., Bodmer, W. F., Bodmer, J., and Trowsdale, J.: Six HLA-D region a chain genes on human chromosome 6: Polymorphisms and associations of DCα-related sequences with DR types.Proc. Natl. Acad. Sci. U.S.A. 81: 3461–3465, 1984
Sundström, C. and Nilsson, K.: Establishment and characterization of a human histocytic lymphoma cell line (U-937).Int. J. Cancer 17: 565–577, 1976
Szoka, F., Jr. and Papahadjopoulos, D.: Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse phase evaporation.Proc. Natl. Acad. Sci. U.S.A. 75: 4194–4198, 1978
Tanno, M., Ikeda, H., and Katagiri, M.: Immunochemical analysis of a novel human la-like antigen distinct from DR- and DR-related supertypic antigen.Jpn J. Transplant. 19: 51–58, 1984
Trowsdale, J., Lee, J., Carey, J., Grosveld, F., Bodmer, J., and Bodmer, W. F.: Sequences related to HLA-DR a chain on human chromosome 6: Restriction enzyme polymorphism detected with DCa chain probes.Proc. Natl. Acad. Sci. U.S.A. 80: 1972–1976, 1983
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Nakatsuji, T., Inoko, H., Ando, A. et al. The role of transfectedHLA-DQ genes in the mixed lymphocyte reaction-like condition. Immunogenetics 25, 1–6 (1987). https://doi.org/10.1007/BF00768826
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DOI: https://doi.org/10.1007/BF00768826