, Volume 18, Issue 3, pp 167–172 | Cite as

Efficient expression of pro-urokinase by human lymphoblastoid Namalwa KJM-1 cells using Moloney retroviral promoter

  • Mitsuo Satoh
  • Hiromasa Miyaji
  • Tatsunari Nishi
  • Tamio Mizukami
  • Seiji Sato
  • Seiga Itoh
  • Mamoru Hasegawa


We have compared the level of expression of several enhancer/promoters in human lymphoblastoid Namalwa KJM-1 cells when fused to a common reporter gene. A cassette containing the pro-urokinase (pro-UK) coding sequence followed by the rabbit β-globin and simian virus 40 (SV40) 3′ nontranslated region was used for evaluation of the enhancer activity. Cells containing Moloney murine leukemia virus (Mo-MuLV) promoter had an average of 10–20 fold higher expression levels of pro-UK than those containing other promoters, such as SV40 early gene promoter, human cytomegalovirus (hCMV) major immediate-early gene promoter, Rous sarcoma virus (RSV) promoter and chicken β-actin gene promoter. The expression level of pro-UK under the control of Mo-MuLV promoter was 2–3 μg/106 cells/day and was constant for more than 6 months. Furthermore, the production of a high producer clone, obtained by using dhfr gene coamplification, reached 30–40 μg/106 cells/day. Thus, Mo-MuLV promoter showed the desired characteristics for efficient expression of foreign genes in Namalwa KJM-1 cells.

Key words

efficient gene expression Moloney retroviral promoter Namalwa KJM-1 pro-urokinase 



dihydrofolate reductase


granulocyte colony-stimulating factor


human cytomegalovirus


long terminal repeat


Moloney murine leukemia virus






Rous sarcoma virus


simian virus 40




thyroid-hormone responsive element


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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Mitsuo Satoh
    • 1
  • Hiromasa Miyaji
    • 1
  • Tatsunari Nishi
    • 1
  • Tamio Mizukami
    • 1
  • Seiji Sato
    • 1
  • Seiga Itoh
    • 1
  • Mamoru Hasegawa
    • 1
  1. 1.Tokyo Research LaboratoriesKyowa Hakko Kogyo Co.Machida-shi, TokyoJapan

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