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Production of monoclonal antibodies and development of an ELISA for solamargine

Abstract

The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibodies against solamargine was produced by fusing splenocytes immunized with a solamargine-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. Extensive cross-reaction of anti-solamargine antibodies against solasonine appeared. Aglycone of solamargine, solasodine cross-reacted with anti-solamargine antibodies resulting in a 43.8% cross-reaction. Insignificant cross-reaction appeared with tomatine (2.06%). The full measuring range of the assay extends from 57.5 pmol ml−1 to 11.5 nmol ml−1 of solamargine.

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Ishiyama, M., Shoyama, Y., Murakami, H. et al. Production of monoclonal antibodies and development of an ELISA for solamargine. Cytotechnology 18, 153–158 (1995). https://doi.org/10.1007/BF00767762

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  • DOI: https://doi.org/10.1007/BF00767762

Key words

  • steroidal alkaloid
  • Solanum khasianum
  • solamargine
  • ELISA
  • monoclonal antibody
  • qualitative analysis
  • mass spectrometry