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Production of monoclonal antibodies and development of an ELISA for solamargine

Cytotechnology volume 18, pages 153–158 (1995)Cite this article

Abstract

The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibodies against solamargine was produced by fusing splenocytes immunized with a solamargine-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. Extensive cross-reaction of anti-solamargine antibodies against solasonine appeared. Aglycone of solamargine, solasodine cross-reacted with anti-solamargine antibodies resulting in a 43.8% cross-reaction. Insignificant cross-reaction appeared with tomatine (2.06%). The full measuring range of the assay extends from 57.5 pmol ml−1 to 11.5 nmol ml−1 of solamargine.

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Author notes

  1. Hideo Shinohara

    Present address: Central Research Laboratories, Kissei Pharmaceutical Co. Ltd., 399-65, Matsumoto-city, Nagano Prefecture, Japan

Authors and Affiliations

  1. Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, 812, Fukuoka, Japan

    Masato Ishiyama, Yukihiro Shoyama & Hideo Shinohara

  2. Graduate School of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812, Fukuoka, Japan

    Hiroki Murakami

Authors
  1. Masato Ishiyama
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  2. Yukihiro Shoyama
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  3. Hiroki Murakami
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  4. Hideo Shinohara
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Ishiyama, M., Shoyama, Y., Murakami, H. et al. Production of monoclonal antibodies and development of an ELISA for solamargine. Cytotechnology 18, 153–158 (1995). https://doi.org/10.1007/BF00767762

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  • DOI: https://doi.org/10.1007/BF00767762

Key words

  • steroidal alkaloid
  • Solanum khasianum
  • solamargine
  • ELISA
  • monoclonal antibody
  • qualitative analysis
  • mass spectrometry