Biotechnology Techniques

, Volume 10, Issue 2, pp 127–132 | Cite as

An enzyme-linked immunoassay for the levansucrase ofZymomonas mobilis using specific antibodies produced against the cloned enzyme

  • Ju-Won Kwak
  • Young-Gyo Seo
  • Ki-Bang Song
  • Sang-Ki Rhee
Article

Summary

Levansucrase gene (levU) ofZ. mobilis had been cloned and overexpressed inEscherichia coli (Song, K.-B. and Rhee, S.-K. (1994)Biotechnol. Lett. 16, 1305–1310). The levansucrase was purified and specific antibodies against it were raised in rabbits. Specificity of the antibodies was demonstrated by Western analysis. By using the antibodies, an efficient, enzyme-linked immunosorbent assay (ELISA) amenable to immunochemical analysis of the levansucrase has been developed. The working range of levansucrase concentration in the developed ELISA was between 75 and 300 ng/ml. The assay was very sensitive to detect the levansucrase as little as 37 ng/ml.

Keywords

Enzyme Immunosorbent Assay Specific Antibody Western Analysis Immunochemical Analysis 

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Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • Ju-Won Kwak
    • 1
  • Young-Gyo Seo
    • 1
  • Ki-Bang Song
    • 2
  • Sang-Ki Rhee
    • 2
  1. 1.Protein Engineering Research GroupKorea Research Institute of Bioscience and Biotechnology, KISTYuseong, TaejonKorea
  2. 2.Applied Microbiology Research GroupKorea Research Institute of Bioscience and Biotechnology, KISTYuseong, TaejonKorea

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