Abstract
Sediment cores, including the associated lake water, were collected from a shallow hypereutrophic lake located in central Florida. Alkaline phosphatase activity (APA) was measured as an indicator of potential organic P mineralization. In both the sediment and water columns, APA was mainly associated with particulate matter; < 10% of APA was within the soluble phase. This suggests that for enzymatic hydrolysis to occur the hydrolyzable organic compounds must be in close proximity to the particle-bound enzyme complex. Both total P (TP) and APA decreased with depth in the sediment, whereas soluble reactive P increased in the 20–40 cm fraction. Resuspension of surficial sediments resulted in an immediate increase in APA, total suspended solids, TP, total Kjeldahl N, and total organic C within the overlying water column. However, these concentrations decreased rapidly following cessation of turbulence and settling of the sediments, emphasizing the close association of these parameters with the sediment.
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References
American Public Health Association (APHA), 1985. Standard methods for the examination of water and wastewater. 16th edn. American Public Health Association, Washington, D. C. 1268 pp.
Ayyakkannu, K. & D. Chadramohen, 1971. Occurrence and distribution of phosphate solubilizing bacteria and phosphatase activity in marine sediments and Porto Novo. Mar. Biol. 11: 201–205.
Baligar, V. C., R. J. Wright & M. D. Smedley, 1988. Aced phosphatase activity in soils of the Appalachian Region. Soil Sci. Soc. Am. J. 52: 1612–1616.
Burns, R. G., 1986. Interaction of enzymes with soil mineral and organic colloids. In: P. M. Huang & M. Schnitzer (eds), Interactions of Soil Minerals with Natural Organics and Microbes. SSSA Spec. Publ. no. 17: 429–451.
Coleman, J. E. & P. Gettins, 1983. Alkaline phosphatase, solution, structure, and mechanism. In: A. Meister (ed.), 381–452. Advances in Enzymology Vol. 55. Wiley.
Degobbis, D., E. Homme-Maslowska, A. A. Orio, R. Donazzolo & B. Pavoni, 1984. The role of alkaline phosphatase in the sediments of Venice Lagoon on nutrient regeneration. Estuar. coast. shelf Sci. 22: 425–437.
Dorich, R. A., D. W. Nelson & L. E. Sommers, 1985. Estimating algal available P in suspended sediments by chemical extraction. J. envir. Qual. 14: 400–405.
Gächter, R. & A. Mares, 1985. Does settling seston release soluble reactive phosphorus in the hypolimnion of lakes? Limnol. Oceanogr. 30: 364–371.
Healey, F. P. & L. L. Hendzel, 1979. Fluorometric measurement of alkaline phosphatase activity in algae. Freshwat. Biol. 9: 429–439.
Heath, R. T. & G. D. Cooke, 1975. The significance of alkaline phosphatase in a eutrophic lake. Verh. int. Ver. Limnol. 19: 293–304.
Holdren, G. C., Jr. & D. E. Armstrong, 1980. Factors affecting phosphorus release from intact lake sediment cores. Envir. Sci. Technol. 14: 79–87.
Juma, N. G. & M. A. Tabatabai, 1978. Distribution of phosphomonoesterases in soils. Soil Sci. 126: 101–108.
Kobori, H. & N. Taga, 1979. Occurrence and distribution of phosphatase in neritic and oceanic sediments. Deep Sea Res. 26: 799–808.
Lee, G. F., 1970. Factors affecting the transfer of materials between water and sediments. Univ. of Wisconsin. Eutrophication Information Program, Literature Review No. 1. Madison, Wisconsin.
Lee, G. F., W. C. Sonzogni & R. D. Spear, 1977. Significance of oxic vs anoxic conditions for Lake Mendota sediment phosphorus release. In: H. L. Golterman (ed.), Interactions between sediments and freshwater. Dr W. Junk, The Hague: 294–306.
McQueen, D. J., D. R. S. Lean & M. N. Charlton, 1986. The effects of hypolimnetic aeration on iron-phosphorus interactions. Wat. Res. 20: 1129–1135.
Moore, P. M. Jr., K. R. Reddy & D. A. Graetz, 1991. Phosphorus geochemistry in the sediment-water column of a hypereutrophic lake. J. envir. Qual. 20: 869–875.
Newman, S., 1991. Bioavailability of organic phosphorus in a shallow hypereutrophic lake. Ph.D. dissertation, Univ. Florida, Gainesville, Fl. 180 p.
Pettersson, K. & M. Jansson, 1978. Determination of phosphatase activity in lake water — a study of methods. Verh. int. Ver. Limnol. 20: 1226–1230.
Pollman, C. D., 1983. Internal loading in shallow lakes. Ph.D. dissertation, Univ. Florida, Gainesville, Fl. 191 pp.
Pomeroy, L. R., E. E. Smith & C. M. Grant, 1965. The exchange of phosphate between estuarine water and sediments. Limnol. Oceanogr. 10: 167–172.
Pulford, I. D. & M. A. Tabatabai, 1988. Effect of waterlogging on enzyme activities in soils. Soil Biol. Biochem. 20: 215–219.
Reddy, K. R. & M. F. Fisher, 1990. Sediment resuspension effects on phosphorus fluxes across the sediment-water interface: Laboratory microcosm studies. Final Report submitted to the South Florida Water Management District, West Palm Beach, FL.
Reddy, K. R. & D. A. Graetz, 1990. Internal nutrient budget for Lake Apopka. St. Johns River Water Management District. Project no. 15-150-01-SWIM. Palatka, Florida. 125 pp.
Rojo, M. J., S. G. Carcedo & M. P. Mateos, 1990. Distribution and characterization of phosphatase and organic phosphorus in soil fractions. Soil Biol. Biochem. 22: 169–174.
Ryding, S-O. & C. Forsberg, 1977. Sediments as a nutrient source in a shallow polluted lake. In: H. L. Golterman (ed.) Interactions between sediments and freshwater. Dr W. Junk, The Hague: 227–234.
Sayler, G. S., M. Puziss & M. Silver, 1979. Alkaline phosphatase assay for freshwater sediments: Application to perturbed sediment systems. Appl. envir. Microbiol. 38: 922–927.
SAS Institute Inc., 1985. SAS user's guide: statistics, SAS Institute Inc., Cary, N.C.
Schindler, D. W., R. Hesslein & G. Kipphur, 1977. Interactions between sediments and overlying waters in an experimentally eutrophied Precambian Shield Lake. In: H. L. Golterman (ed.), Interactions between sediments and freshwater. Dr W. Junk, The Hague: 235–243.
Speir, T. W. & D. J. Ross, 1978. Soil phosphatases and sulphatases. In: R. G. Burns (ed.), Soil Enzymes. Academic Press, New York: 197–249.
Stumm, W. & J. O. Leckie, 1970. Phosphate exchange with sediments; its role in the productivity of surface waters. Fifth Int. Water Polln. Res. Conf. III: 2611–2616.
Tabatabai, M. A. & J. M. Bremner, 1969. Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol. Biochem. 1: 301–307.
Tessenow, U., 1972. Losungs-, Diffusions- und Sorptionsprozesse in der Oberschicht von Seesedimenten. 1. Ein Langzeitexperiment unter aseroben und anaeroben Bedingungen in Fleibgleichgewicht. Arch. Hydrobiol. Suppl. 38: 353–398.
U. S. Environmental Protection Agency (USEPA), 1979. Environmental impact statement. Lake Apopka restoration project. Lake and Orange Counties, Florida. (EPA 904/08–79–043). U. S. Environmental Protection Agency, EMSL, Cincinnati, OH.
Walker, T. W. & A. F. R. Adams, 1958. Organic phosphorus. In: A. L. Page, R. H. Miller & D. R. Keeney (eds), Methods of soil analysis, Part 2: Chemical and microbiological properties. 1982 ASA, SSSA. Madison, WI: 411–413.
Wolanski, E., T. Asaeda & J. Imberger, 1989. Mixing across a lutocline. Limnol. Oceanogr. 34: 931–938.
Young, T. C., J. V. DePinto, S. C. Martin & J. S. Bonner, 1985. Algal available particulate phosphorus in the Great Lakes basin. J. Great Lakes Res. 11: 434–446.
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Newman, S., Reddy, K.R. Sediment resuspension effects on alkaline phosphatase activity. Hydrobiologia 245, 75–86 (1992). https://doi.org/10.1007/BF00764767
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DOI: https://doi.org/10.1007/BF00764767