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Pharmaceutical Chemistry Journal

, Volume 2, Issue 2, pp 98–99 | Cite as

Determination of the reactivity of the guanidine group in thermally inactivated insulin

  • M. I. Plekhan
  • P. A. Babich
Article

Conclusions

  1. 1.

    Thermal inactivation of insulin (in particular, fibril formation) does not change the reactivity of the guanidine group of insulin in the Sakaguchi reaction.

     
  2. 2.

    Sparingly soluble protein samples, in particular, thermally inactivated insulin, can be analyzed for their content of guanidine groups according to the Sakaguchi reaction, by dissolving the sample in the form of the copper biuret complex.

     

Keywords

Copper Organic Chemistry Fibril Soluble Protein Protein Sample 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Literature cited

  1. 1.
    S. Sakaguchi, J. Biochem. (Tokyo),5, 25 (1925).Google Scholar
  2. 2.
    D. F. Waugh, J. Am. Chem. Soc.,66, 2731 (1940).Google Scholar
  3. 3.
    M. I. Plekhan and P. A. Babich, Biofizika,11, 2, 355 (1966).Google Scholar

Copyright information

© Consultants Bureau 1968

Authors and Affiliations

  • M. I. Plekhan
    • 1
  • P. A. Babich
    • 1
  1. 1.All-Union Antibiotic Scientific Research InstituteMoscow

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